RNA-seq研究方法与策略-zzz.pptVIP

Analysis Stereotypical RNA-seq Analysis Pipeline Demultiplex, filter, and trim sequencing reads. Normalize sequencing reads (if performing de novo assembly). de novo assembly of transcripts (if a reference genome is not available). Map (align) sequencing reads to reference genome or transcriptome. Annotate transcripts assembled or to which reads have been mapped. Count mapped reads to estimate transcript abundance. Perform statistical analysis to identify differential expression (or differential splicing) among samples or treatments. Perform multivariate statistical analysis/visualization to