真核生物基因调控.pptVIP

（1） DNA-binding domains The helix-turn-helix domain The zinc finger domain The basic domain The helix-turn-helix domain（HTH） The domain often contain 20 amino acids, consisting of two α-helix and separated by a β–turn. One of the α-helix is named recognition helix, because it include several amino acids to directly recognizing the major groove DNA sequence. The other contacts with the backbone of DNA sequence and has no specificity to DNA sequence. The binding rely on the hydrogen bond between the side chain of protein and specific base pairs of DNA sequence. HTH (helix-turn-helix) α-helix (N-terminus)----specific α-helix (C-terminus)----non-specific Proteins contain this domain Antennapedia transcription of Drosophila Homeodomain（果蝇同源异型域） Other proteins Phage λ cro repressor Lac and trp repressor cAMP receptor protein (CRP) helix-turn helix，HTH 3.4nm 2 1 3 + 2 1 3 2 1 3 2 1 3 2 1 3 2 1 3 ?-phage Cro The zinc finger domain This domain consist 30 amino acids. The four amino aicds(4 Cys/ 2Cys+2His) bind to Zn ion. Coordination bond（配位键） Type C2H2 zinc finger C4 zinc finger C2H2 zinc finger C2H2 zinc finger 转录因子SP1 （GC盒） 、连续的3个锌指重复结构。 Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn Zn The basic domain The basic domain is generally associated with one or other of two dimerization domains, the leucine zipper or the helix-loop-helix (HLH) motif. These are referred to as basic leucine zipper (bZIP) or basic HLH proteins. Dimerization of the proteins brings together two basic domains which can then interact with DNA. 由两段α-螺旋平行排列构成的二聚 化结构域，其每个α-螺旋中存在每7个残基规律性排列的Leu残基，两排亮氨酸残基疏水侧链排列成拉链状形成疏水键使蛋白质结合成二聚体，N端肽段借Arg,Lys侧链基团与DNA的碱基互相结合而而实现蛋白质与DNA的特异结合。 basic leucine zipper (bZIP) basic leucine zipper，bZIP COOH NH2 COOH NH2 1 8 15 22 29 36 1 8 15 22 29 36 Leucine residue C N C O H CH2 CH CH3 CH3 N端20～30个富含碱性氨基酸结合DNA 这类蛋白质的DNA结合结构域实际是以碱性区和亮氨酸拉链结构域整体作为基础的。 思考题： 你克隆到的一个蛋白质，序列如下： 001 MESADFYEVE PRPPMSSHLQ SPPHAPSNAR LWLSPGRGPR AAPSPTCRPG AAGRICEHET 061 SIDISA