茶树花多糖免疫调节与抗肿瘤活性的研究.pdfVIP

生物化学与生物物理进展 Progress in Biochemistry and Biop hys ics 2010, 37(6): 646~653 Immunomodulatory and ntitumor ctivity of 孕olysaccharide 陨solated 云rom 栽ea 孕lant 云lower * HAN Quan, LING Ze-Jie, HE Pu-Ming , XIONG Chang-Yun (Dep artment of Tea Science, Zhej iang Univ ers ity, Hangz hou 310029, China) Abstract Tea plant flower is a product of the tea plant during its growing. Polysaccharide are abundant in tea flower. However, the components in tea flower are underutilized at present. The study aimed at evaluating the antitumor activity and immunomodulatory effect of tea plant flower polysaccharide (TFP) v ia various in v ivo assay systems. The inhibition effect of TFP on sarcoma 180 tumor (S180)-bearing mice was observed at dosages of 75, 150 and 300 mg/kg by systematically to measure the S180 tumor inhibition rate, mice survival rate and cellular immunity. The result showed that continuous administration of TFP for 10 day continuously was found to inhibit the growth of transplanted S180, prolong the mice survival days, promote the plasma interleukin-2, interferon- levels and improve the T-lymphocyte subsets CD4+ + + and CD4 /CD8 percentages after treatment with TFP. In addition, TFP was found to increase the delayed-type hypersensitivity response and macrophage phagocytosis significantly. These results strongly suggest that TFP enhances the host defense response to tumor due in part to the immunomodulatory activity. Key words antitumor activity, immunomodulatory, delayed-type hypersensitivity, carbon particle cl