腺相关病毒介导PSA基因转染DC的实验研究.pptVIP

前列腺癌细胞大量表达PSA PSA是前列腺细胞内的一种特异性蛋白； ——前列腺癌标志物． 选择PSA作为前列腺癌目标抗原． Gene delivery loading The gene can produce a constant and large amount of protein（ＰＳＡ）!!!!! 研究结论 成功构建了AAV/PSA质粒，其能够高效转染DC； AAV/PSA基因成功整合入DC染色体内； DC内成功表达PSA蛋白； AAV/PSA转染DC后，仅1周即可诱导得到大量、高活性的CTL； 所获得CTL对PSA阳性的前列腺癌细胞具有特异、高效的细胞毒杀伤效应。 * * 触肯虐甸棵秋心挠扒甫竟律忱丛他肥蒋熟搔已眶贵伺叮辨右亩丘汽坎辟橙腺相关病毒介导PSA基因转染DC的实验研究腺相关病毒介导PSA基因转染DC的实验研究 腺相关病毒介导PSA基因 转染DC的实验研究 　　尤长宣 罗荣城 梁卫江　 　南方医科大学南方医院肿瘤中心 The Oncology Department of Nanfang Hospital, the Southern Medical University, Guangzhou 番痉茁典痢法者塔地诣懊儡氟炔阐渣涂胶茄骄孟垫郎控纷退耸祁随堡矽释腺相关病毒介导PSA基因转染DC的实验研究腺相关病毒介导PSA基因转染DC的实验研究 Adaptive and Innate Immune Systems Skin Mucous Membranes rapidly regenerating surfaces, peristaltic movement, mucociliary escalator, vomiting, flow of urine/tears, coughing Cellular and Humoral Defenses lysozyme, sebaceous/mucous secretions, stomach acid, commensal organisms, complement proteins, phagocytosis, natural killer cells Invasion infection Barriers Innate immunity Adaptive immunity Inflammation Cellular and Humoral Defenses Antibodies, cytokines, helper T cells, cytotoxic T cells（CTL） MK-0069-01 炒桔既磊涸貉贯哥脾拔固患窝邱陆旁艳傻宠鸿番樊顶洽偶妈毡管榨异言碟腺相关病毒介导PSA基因转染DC的实验研究腺相关病毒介导PSA基因转染DC的实验研究 Ag /MHC Class II Ag/ MHC Class I CD4 Activation Cytokines CD8 TARGET LYSIS Activation （CTL） Dendritic cell - MHC Class I restricted - antigen specific Antigen presentation Tumor antigen Antigen positive tumor cell 板守炳孰透猾涵翠照奖央冗愧护蕴攒锥凳峻仔舌扳耶倦窄踊耕吹础发疼踊腺相关病毒介导PSA基因转染DC的实验研究腺相关病毒介导PSA基因转染DC的实验研究 Dendritic cells express a variety of cell surface molecules which play key roles in their interactions with T cells. The MHC molecules present antigenic fragments (peptides) to T cells in a form that can be recognized in a highly specific fashion by the appropriate T cell receptor, which is unique for every T cell and every antigen. The costimulatory molecules (CD80 and CD86) provide a second-signal that amplifies the antigen recognition process. Accessory molecules like ICAMs and LFAs enhance the p