Biolog-ECO解析有机氯农药污染场地土壤微生物.docxVIP

/2013-10-25 DOI: 10.3724/SP.J.1145.2013.00759 / 应用与环境生物学报 Chin J Appl Environ Biol 2013，19 (5): 759-765 Biolog-ECO解析有机氯农药污染场地土壤微生物群落功能多样性特征* 郑丽萍1, 2 龙 涛1, 2** 林玉锁1, 2 于赐刚1, 3 刘 燕1, 3 祝 欣1, 2 （环境保护部南京环境科学研究所 南京 210042） （国家环境保护土壤环境管理与污染控制重点实验室 南京 210042） （国家环境保护生物安全重点实验室 南京 210042） 摘要为考察有机氯农药对受污染场地土壤微生物群落的影响，采用Biolog-ECO技术对有机氯农药污染场地的土壤微生物群落功能多样性进行分析. 结果表明，高水平的有机氯农药污染（DDTs 451.5 mg/kg）显著抑制土壤微生物活性，而较低水平的污染对土壤微生物的生理活性呈现一定的促进作用. 高浓度的有机氯农药污染土壤中，微生物在碳源的利用上显示了较大的差异，对胺类（胺/氨基化合物）不能利用，但对双亲化合物的利用率则有所提高. 不同程度有机氯农药污染土壤中，微生物群落功能多样性也存在显著差异，在无污染或轻度污染的土壤中，微生物总体表现出较高的活性，利用羧酸类和氨基酸相对较多，如D-半乳糖醛酸、衣康酸、L-精氨酸、L-天门冬酰胺；而在污染严重的土壤中，微生物利用氨基酸类及糖类较多，如α-D-乳糖和L-精氨酸. 因此，土壤微生物的生理活性和群落功能多样性可能受污染土壤中有机氯农药残留浓度的影响. 图3 表5 参29 摘 要 关键词有机氯农药；Biolog-ECO；土壤微生物；群落功能多样性；AWCD 关键词 CLC X171.5 : S154.36 Biolog-ECO Analysis of Microbial Community Functional Diversity in Organochlorine Contaminated Soil* ZHENG Liping1, 2, LONG Tao1, 2**, LIN Yusuo1, 2, YU Cigang1, 3, LIU Yan1, 3 ZHU Xin1, 2 (Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing 210042, China) (State Environmental Protection Key Laboratory of Soil Environmental Management and Pollution Control, Nanjing 210042, China) (Key Laboratory on Biosafety of National Environmental Protection, Nanjing 210042, China) Abstract To evaluate the impact of contaminant level on soil microbial communities, the microbia1 community functiona1 diversity in soil collected at an organochlorine pesticides (OCPs) contaminated site was investigated with the Biolog-ECO system. The results showed that high concentration of OCPs in soil (DDTs 451.5 mg/kg) could significantly inhibit soil microbial activities, while low level of OCPs promoted microbial physiological activity. Under high concentration of OCPs, the carbon source utilization behavior of soil microbes exhibited significant differences, as utilization of amine/amino compounds was completely inhibited, while that of amphipathic compounds was improved. Th