石胆酸与液晶乳液的光学检测.docVIP

Optical Detection of Lithocholic Acid with Liquid Crystal Emulsions Tanmay Bera and Jiyu Fang* Department of Materials Science and Engineering,University of Central Florida,Orlando,Florida32826,United States 1.INTRODUCTION Bile acids are physiologically important metabolites which are biosynthesized from cholesterol in the liver and then stored in the gall bladder for the digestion of fatty foods.1It has been found that individuals who su?er from intestinal diseases have an elevated concentration of bile acids at micromolar levels because the hepatic synthesis and clearance of bile acids are disturbed.2Since there is a clear distinction in the concentration of bile acids between healthy and diseased individuals,the concentration level of bile acids has been used as a valuable biomarker for the clinical diagnosis of intestinal diseases.3Chromatography coupled with mass spectrometry is a common method for the detection of bile acids.4?6Although the precision of the chromatography?mass spectrometry method in the detection of bile acids is high,this method is time-consuming and requires expensive instruments.Recently, chemical and optical sensors have been developed for the detection of bile acids in aqueous solution.7?10For example, Koide et al.7reported a chemical sensor based on a three-enzyme system(bile acid sulfate sulfatase,β-hydroxysteroid dehydrogenase,and nicotinamide adenine dinucleotide.The hydrogen peroxide produced from the three-enzyme system was used for the detection of bile acids.Wu et al.9fabricated an optical sensor by?lling a molecularly imprinted hydrogel into a three-dimensional colloidal crystal.The resultant photonic hydrogel with hierarchical porous structures showed optical changes in response to the adsorption of bile acids in the hydrogel.Liu and co-workers synthesizedβ-cyclodextrin(CD derivatives appended with naphthalene and quinoline as ?uorophores,and used them as?uorescence-based sensors for the detection of bile salts.10The transfer