间充质干细胞上清液对细粒棘球绦虫原头节活性的影响.pdfVIP

中文摘要 目的 探讨不同浓度间充质干细胞上清对细粒棘球绦虫原头节活性是否存在影响及浓度差 异性。 方法 1. 从4-6周龄C57 小鼠下肢长骨干内提取培养MSCs； 2. 倒置显微镜下观察MSCs 的细胞形态及生长情况； 3. MSCs 100% 50% 培养、传代并留取培养上清液，稀释成 及 浓度； 4. 从昌吉屠宰场获取自然感染的绵羊羊肝并从中提取细粒原头节，培养观察其形态及 活性； 5. 实验分组使用不同浓度MSC上清液培养原头节，不同时间节点下伊红染色观察计数， 留取标本检测Caspase-3 酶活性，制备电镜标本； 6. 按照Caspase-3 酶试剂盒操作步骤检测标本中酶的活性，电镜下观察不同时间点原头 节的微观形态变化； 7. SPSS 17.0 实验数据处理及统计学分析 （ ）。 结果 1. 间充质干细胞上清液与细粒头节体外培养对其生存率有提升。 2. 间充质干细胞上清降低了体外培养细粒头节Caspase-3 酶的活性。实验组与对照组 Caspase-3 P0.05 酶活性有统计学意义 （ ） 3. 电镜下观察原头节实验组较对照组头节形态更具有完整性，局部结构存在差异。 结论 间充质干细胞上清液能有效维持体外培养的细粒棘球绦虫原头节的活性。 关键词 间充质干细胞；细粒棘球绦虫原头节；活性 I Abstract Objective To investigate the effect of mesenchymal stem cell supernatant of different concentrations on theactivity ofechinococcusgranulosusandtheconcentration difference. Methods 1. Extraction and culture of MSCs from the lower limb long bones of C57 mice aged 4-6 weeksMorphological characteristicsofMSCswereobservedbymicroscopy; 2. Observethemorphology andgrowth ofMSCsunder amicroscope； 3. MSCs were cultured, passaged, and the supernatant was taken and diluted to 100% and 50%concentration； 4. The experimental groups were cultured using different concentrations of MSC supernatantsto culture theprotocephala, and the eosin staining was observed and counted at different time points, Save the specimen to detect the Caspase-3 enzyme activity and preparetheelectronmicroscope specimen； 5. Follow the steps of the Caspase-3 enzyme kit to detect the enzyme activity in the specimen, and observe the micromorphological changes of the protocephala at different timepointsunder an electron microscope; 6. Experimentaldataprocessingand statistical analys