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×××× 2014.9.24 Seminar report paperMicrobial community structure and dynamics of dark fire-cured tobacco fermentation Key words 1. Journal and author 2. Background 3. Process and result 4. Discuss Journal ☆ AEM publishes a substantial share of the most significant current research in the areas of biotechnology, microbial ecology, food microbiology, and industrial microbiology. ☆ Highlighting research findings applicable to the development of new processes or products. ☆ AEM is a broad-based journal that is indispensable to those whose basic work has implications of near-term practical benefit. Author KEY PUBLICATIONS： [1] Di Giacomo, Michele. A stepped approach to vascular access device selection. British journal of nursing 19(19),2010. [2] Di Giacomo, Michele. Comparison of three peripherally-inserted central catheters: pilot study. British journal of nursing 18(1), 2009. [3] Di Giacomo, Michele; Paolino, Marianna; Silvestro, Daniele. Microbial community structure and dynamics of dark fire-cured tobacco fermentation. Applied and Environmental Microbiolog. 73(3),2007. Michele Di Giacomo British American Tobacco Italia, via Cinthia, 80126 Naples, Italy Types of tobacco 1 Aromatic Fire-cured 2 Brightleaf tobacco 3 Burley 4 Cavendish 5 Corojo 6 Criollo 7 Oriental Tobacco 8 Perique 9 Shade tobacco 10 Thuoc lao 11 Type 22 12 White Burley 13 Wild Tobacco 14 Y1 Background Background The dynamics of the process, lasting ca. 18 days, ha never been investigated in detail Tobacco taste Background The V3 region of the 16S rRNA gene was selected to enhance the range of DGGE resolution V4 to V5 region were generated to improve the phylogenetic inference through SSCP analysis. The main body of the article Process and result Physical-chemical profile of dark fire-cured tobacco fermentation. (i) Temperature, pH, and Microbial population; (ii) Sugars, Citric and Malic acids, Nitrate content; (iii) Oxalic acid, Nitrite, Tobacco-specific nitrosamine content(TSNA). Process and