非洲猪瘟快速诊断技术和疫苗研究进展.pdfVIP

吴晓东 Wu Xiaodong 国家非洲猪瘟参考实验室 National reference laboratory for ASF 中国动物卫生与流行病学中心 1 China Animal Health and Epidemiology Center • 非洲猪瘟快速诊断技术研究进展 Research progress in rapid diagnosis of African Swine Fever – 1. 诊断技术综述 A review of diagnostic techniques • 1.1 非洲猪瘟诊断技术 ASF diagnostic techniques • 1.2 快速诊断技术定义及应用范围 Definition(in China) and application scope of rapid diagnostic techniques – 2. 病原检测技术 Pathogen detection technology • 2.1 分子生物学技术 Molecular Biology Technology • 2.2 免疫学技术 Immunological Technology – 1.3 抗体检测技术 Antibody Detection Technology • 非洲猪瘟疫苗研究进展 Research progress of African Swine Fever vaccine – 1. 非洲猪瘟病毒分离 Isolation of African Swine Fever Virus – 2. 基因缺失毒株构建 Development of Gene Deletion Vaccine – 3. 细胞系筛选 Screening of cell line 2 实验室诊断 Laboratory diagnosis 病原学 Antigen 血清学 Antibody 病毒分离 间接ELISA Virus isolation Indirect ELISA 阻断ELISA 直接免疫荧光法 Blocking ELISA Direct IFC 免疫印迹 IB 普通PCR PCR 荧光PCR Real-time PCR 免疫荧光 Immunofluorescence