血液DNA快速提取方法.docVIP

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4oC for Long Period Materials and Methods Standard chemicals This method uses standard chemicals that can be obtained from any major supplier; we used chemicals supplied by Sigma Co. as follow: EDTA (0.5 M), pH 8.0: Add 186.1 gr of anhydrous EDTA to 800 ml of distilled water. Adjust pH to 8.0 with NaOH pellets. Make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 15 min. 1 M Tris-HC1, pH 7.6: Dissolve 121.1 gr of Tris base in 800 ml of distilled water. Adjust pH with concentrated HCl. Allow mixture to cool to roo