化学实验操作 色谱概论 2.Chromatography.pptVIP

LOGO Chromatography Chromatography is the science of physical separation of species in a mixture. It may be used to measure components of both qualitatively and quantitatively. The basis of all chromatographic techniques is the principal of retention. That under certain conditions molecules move at different rates through a separating bed. Two main features Mobile phase Liquid or gas Move Carry the mixture components Stationary phase Solid or immobilised liquid material Not move Separation Process DESORBED ADSORBED ADSORBED Components are adsorbed onto the surface of the stationary phase Components desorb back into the mobile phase Components are adsorbed onto the surface of the stationary phase Example The white molecules are more strongly adsorbed and become left behind by the blue ones Qualitative: separate collect Chromatography Quantitative: with a analytical device Four types of chromatography CHROMATOGRAPHY Four types Adsorption Size exclusion Partition Ion Exchange TLC, Column chromatography Paper chromatography, GC Ion exchange resin Porous sieve Polarity In adsorption and partition chromatography Polarity of components determines The strength of attraction for the stationary phase Solubility in the mobile phase Polarity of compounds RH RX RNO2 ROR, RCOOR, RCOR, RCHO RCONHR RNH2 ROH H2O RCOOH Polarity of solvents petroleums cyclohexane trichloroethane toluene ether ethyl acetate acetone C3H7OH C2H5OH H2O Column chromatography Paper chromatography Thin lay chromatography High performance liquid chromatography Gas chromatography /v_show/id_XMTU0ODQ2NjAw.html * * * .au/bhi2/toolbox5/Laboratory/StudyNotes/snWhatsChromatography.htm LOGO /v_show/id_XMTU0ODQ2NjAw.html * * * .au/bhi2/toolbox5/Laboratory/StudyNotes/snWhatsChromatography.htm