分光光度法测定巯基含量.pdf

T E C H N I C A L N O T E S Spectrophotometric Method for Determination of Heat-Activated Sulfhydryl Groups of Skimmilk Abstract 8.0, and 4 ml of distilled water were added. The lots were agitated and two grains of am- By suitable modification, adaptation was monium sulfate added. The samples were again made of the 5,5-dithiobis (2-nitrobenzoic shaken vigorously and the contents filtered acid) reagent method for the spectropho- tometric determination of activated sulfhy- dryl ( - - S H ) groups of heated skimmilk. 12 The modifications consisted of using a nitrogen-purged system, controlling pH, adding ammonium sulfate, filtering, and I(~ determining absorbance of the filtrate at 412 mlz. A standard cysteine-HC1 curve -.i was prepared in a similar manner. The E 08 modified procedure yielded results consis- _¢2 tent with other cmTently used methods. The activation of - - S H groups in heated ~ 06 skimmilk, as determined by the modified Q • procedure, followed the kinetics of a first order reaction with reaction rate constants o 0.4 (kn, l._l) of 0.078, 0.142, 0.384, and 0.805