免疫组库测序技术介绍[借鉴].pptVIP

The Immune System * 精品PPT·借鉴参考 Innate immune system a general response to anything other than recognized “self cells” macrophages - able to ingest small foreign invaders Mast cells /basophils release histamine that dilates blood vessels causes redness * 精品PPT·借鉴参考 Adaptive immune system a specific counter-assault against a “known foreign” invader [previously recognized] * 精品PPT·借鉴参考 Key-lock Every mature T cell (B cell) expresses a single TCR (BCR) with specificity for a limited number of antigens. T cell receptor * 精品PPT·借鉴参考 How many T cells in your body? 1. Human blood has about one million T cells in every milliliter (ml). 2. The average adult human has about 10 liters of blood. 3. There are 10,000 ml in 10 L. 4, So, there are about one million x 10,000 T cells in the bloodstream. This comes to 1010. About 1/50 of all T cells are found in the blood. Most of the rest are in the spleen and lymph nodes. Some are also found in the bone marrow, liver, and other tissues. 5. So, I would estimate the number of T cells in the human body to be about 5 x 1011. * 精品PPT·借鉴参考 B lymphocyte development How antibody diversity is generated Janeway C., Immunobiology, 2004 Stem cell Chromosome 14, heavy chain * 精品PPT·借鉴参考 B lymphocyte development How antibody diversity is generated Janeway C., Immunobiology, 2004 Stem cell Chromosome 14, heavy chain Pro-B cell * 精品PPT·借鉴参考 B lymphocyte development How antibody diversity is generated Janeway C., Immunobiology, 2004 Stem cell B cell Chromosome 14, heavy chain Pro-B cell * 精品PPT·借鉴参考 Antibody repertoire estimates Joint diversity 3-5bp random insertion/deletion Theoretical calculation: Immune system only uses 83 gene Segment to code 1014 BCR sequences * 精品PPT·借鉴参考 Diversity and healthy Intrinsic factors that influence the expression of the TCR repertoire in humans: human MHC (HLA) system and TCR gene polymorphisms, Extrinsic factors: environmental factors, such as infection and superantigens. In normal indivi