2013年东南大学医学院研究生复试面试试题 综合组(基础.pdfVIP

J Clin Immunol (2013) 33:368–377 DOI 10.1007/s10875-012-9821-x ORIGINAL RESEARCH Expression and Function of Dectin-1 is Defective in Monocytes from Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis Claudia Salazar-Aldrete Marta Galán-Díez Elena Fernández-Ruiz Perla Niño-Moreno Lizbeth Estrada-Capetillo Carlos Abud-Mendoza Esther Layseca-Espinosa Lourdes Baranda Roberto González-Amaro Received: 10 July 2012 /Accepted: 12 October 2012 /Published online: 25 October 2012 # Springer Science+Business Media New York 2012 Abstract The aim of this work was to study the expression defective function of dectin-1. Our data suggest that dectin-1 and function of the innate immune receptor dectin-1 in patients receptor defects could contribute to the pathogenesis of auto- with systemic lupus erythematosus (SLE) and rheumatoid ar- immune inflammatory conditions. thritis (RA). We studied twenty-six patients with SLE not receiving immunosuppressive therapy, twenty-six patients with Keywords Dectin-1 . monocytes . systemic lupus RA, and fifteen controls. We found that monocytes from SLE erythematosus . rheumatoid arthritis patients showed a diminished expression of dectin-1 compared to healthy controls, and an inverse correlation between percent of dectin-1+ cells and the disease activity score was detected. Introduction In addition, cells from SLE patients showed an abnormal calcium flux response induced by dectin-1 ligands as The innate immunity is the first line of defense against well as an enhanced release of IL-1 β, IL-6 and TNF-α, pathogens. A growing family of receptors, known as PRR ’s but not IL-23, upon dectin-1 engagement. Monocytes from (pattern recognition receptors), has an important role in the patients with RA also showed a diminished expression,