生物分离工程-双语版-3教学内容.ppt

生物分离工程-双语版-3;II Chromatography procedures ;A gel filtration chromatography;Exmaple 3.1 carboxylesterase purification ;Exmaple 3.1 carboxylesterase purification ;Difference between crude and purified esterase forms;;Results and discussion about table 3.1 ;Purification of antigenized immunoglobulins with monomethoxypolyethylene glycol;Purification of antigenized immunoglobulins with monomethoxypolyethylene glycol;; Example 3.2 Tissue plasminogen  activator purification ;tPA purification protocols;;;t-PA from CHO and E.coli;Xylanase purification ;;Purification of two isoenzymes of xylanase from streptomyces;Purification of highly thermostable glucose isomerase;;Example 3.3 purification of two endo-β-glucanase from aerobic fungus;;Purification protocol for β-glucosidase from clostridium thermocellum ;;Pectin methylesterase purification;;Hydroxyapatite chromatography;;工艺条件;B Hydroxylapatite chromatography;Chromatography media ;硅胶;Sorbitol and sorbitol dehydrogenase;Purification of D-xylulokinase ;New words and vocabularies;New words and vocabularies;New words and vocabularies ;New words and vocabularies ;Questions ;;Hydrophobic chromatography;疏水层析;疏水层析介质制备过程示意图 ;工艺条件;New words and vocabularies ;Affinity chromatography ;Cluster model of multivalent affinity; 亲和层析; 基质活化方法与配基偶联 ;活化方法（1）;溴化氰活化与配基偶联化学反应式 ; 活化方法（2）;环氧氯丙烷活化反应式 ; 环氧基的氨化及偶联配基反应 ;Ion-exchange chromatography;离子交换法;;强酸性阳离子交换树脂;弱酸性阳树脂;强碱性阴树脂;弱碱性阴树脂;Example 3.11 Production of blood protein using ion-exchange ;离子交换提取蛋白质;亲水性离子交换剂功能团;离子交换剂的交换容量;吸附机理;蛋白质的离子交换层析;;;;;New words and vocabularies;New words and vocabularies;III Crystallization ;Example 3.12 purification and crystallization of lipase ;Purification and crystallization of lipase ;Antibiotics crystallization;Antibiotics crystallization;IV other techniques ;Example 3.17 purification of β-glucosidase;;Example 3.18 separation of peroxidase ;;;;V Conclusions ;New words and vocabularies;New words and vo