生物分离工程-双语版-5教案资料.ppt

生物分离工程-双语版-5;Denaturation ;Adsorption isotherm;II chromatography techniques;II chromatography techniques;A Ion-exchange chromatography;Proteins retention model;Displacement mode chromatography ;;Purification of monoclonal IgM from hybridoma ;Silica-based ion-exchange column;Poly (aspartic acid)-silica IEC;;Example 5.1 Separation of using HPIEC;;Example 5.2 Separation of mRNAs;Purification of X-prolyl dipeptidyl peptidase ;New words and vocabularies (1) ;New words and vocabularies (2);Purification of aminopeptidase ;Separation of lipase;Example 5.4 separation of (bFGF) and alkaline phosphatase; Purification of bFGF; Purification of β-glucosidase ;Antibiotics ;Microheterogeneity ;Mechanistic considerations;Reversed-phase HPLC ;Reversed-phase HPLC ;Example 5.6 protein separation using conformational differences;New words and vocabularies (3);D Mechanistic Considerations;Two-state denaturation model;Example 5.7 kinetics of denaturation on a surface in RP-HPLC;Kinetics of denaturation;;Reversible denaturation model;Slurry-packed fuse-sillca microcolumns;Example: protein denaturation by using Synthetic peptide polymer during RP-HPLC;E Hydrophobic interaction chromatography ;HIC process ;Thermal behavior of standard proteins in HIC ;HIC selectivity ;Example 5.8 scale-up of HIC purification of antitumor antibiotic SN-07;F mechanistic considerations;G Subunit exchange chromatography;Subunit exchange chromatography;Example 5.10 immunoaffinity purification of antigen (sCDS);Example 5.11 Purification of α-amylase by immunoaffinity chromatography with cross-reactive antibody;H other chromatography techniques;Centrifugal partition chromatography (CPC) ;;CPC process advantage;Conclusions ;New words and vocabularies;New words and vocabularies;New words and vocabularies (2);Questions ;此课件下载可自行编辑修改，仅供参考！感谢您的支持，我们努力做得更好！谢谢