生物分离工程英文课件电子教案.ppt

生物分离工程英文课件;§1.1 Downstream processing in biotechnology;;;§1.2 Demands on the  downstream processing;;§1.3 Separation mechanism and unit operation ;;§1.4 Estimation for separation efficiency ;;;Chapter 2 Solid-liquid separation and  cell disruption ;§2.1 Cell separation;§2.1.1 Settling;;§2.1.2 Centrifugation ;;;;§2.1.3 Filtration;;;§2.2 Cell disruption;;§2.2.1 Cell structures ;§2.2.2 The principles of cell disruption;§2.2.3 The means of cell disruption;;;;;;;A combination of enzymatic/chemical lysis with mechanical disintegration has been suggested in enhancing the efficiencies of the respective methods, with savings in time and energy and the facilitation of subsequent processing.;;;;Chapter 3 Precipitation;;;§3.1 Salting-out precipitation ;§3.1.1 Theory ;§3.1.2 The influencing factors; temperature and pH（to ?） under a higher ionic strength the solubility of proteins will decrease accompanied with the increase of temperature.;§3.1.3 Unit operation of salting-out;; plot a figure to describe the correlation between the concentration of total protein and that of given protein and saturation of (NH4)2SO4 in the mother liquor，by that decide the additive amount based on the request for recovery of products.;§3.1.4 Application;§3.2 Isoelectric precipitation;;As a rule it is applied to precipitation of hydrophobic proteins e.g. casein, and not suitable for hydrophilic proteins e.g. glutin. So applying fields is not wider than salting-out precipitaion. Characteristic： advantage：it is facilitated to subsequent operation； disadvantage：sometimes extremes of pH denature the products.;§3.3 Organic solvent precipitation;;§3.4 Another methods;§3.4.1 Thermal precipitation;§3.4.2 Special agents;;Chapter 7 Affinity purification;;§7.1 Basic principle;§7.1.1 Molecular recognition processes;§7.1.2 Receptor-ligand interactions;§7.2 Affinity chromatography;§7.2.1 Principle and op