烟酰胺干预uvb诱导豚鼠皮肤色素沉着的作用.docxVIP

烟酰胺干预UV瞬导豚鼠皮肤色素沉 着的作用 【摘要】目的探讨烟酰胺对中波紫外 线致豚鼠皮肤色素沉着的抑制作用。方法 以亚红斑剂量UVB照射豚鼠背部10天建立 皮肤无急性损伤色素沉着动物模型后， 外涂 基质和％ 5% 10%勺烟酰胺4周，在此期间 观察肤色改变和测定皮肤 L值。涂药结束后 取材，进行Masson— Fontana、冰冻切片 Dopa 染色和黑素细胞超微结构观察，观察黑素细 胞数量、形态和功能的改变。结果 涂抹5% 烟酰胺4周后，豚鼠皮肤变白，L值显着下 降，多巴阳性黑素细胞数及黑素含量指数较 对照组明显减少。结论5 %烟酰胺对UVB秀 导的色素沉着有明显的抑制， 它主要是通过 抑制黑素细胞的增殖和黑素细胞内黑素小 体的合成而发挥作用。 【关键词】 烟酰胺；色素沉着； 黑素细 胞 [Abstract 】Objective To investigate the inhibitory effect of nicotinamide on hyperpigmentation induced by UVB. Methods After hyperpigmentation was induced by UVB radiation on the back of brown guinea pigs , substance , %, 5%and 10% nicotinamide were applied on the exposed skin for 4 weeks respectively. During this process , the changes of skin coloration were observed with naked eyes and L value were measured. Then the biopsy specimens were taken from the treated skin , sections were prepared for special staining and TEMexamination and the number of melanocytes and melanin content index(MCI) were determined. Results After the application of 5% nicotinamide for 4 weeks, lightening effect was obviously observed with naked eyes and the L value decreased significantly. Compared with the substance control , the number of Dopa positive melanocytes and melanin content index were all significantly reduced () in the treated skin with 5% nicotinamide. Conclusion 5% nicotinamide is effective in lightening the UVB-induced pigmentation of guinea pig skin. This effect may be related to the declined proliferation of melanocytes and the inhibition of melanin synthesis in melanocytes. 【Key words 】nicotinamide ; pigmentation ； melanocyte 烟酰胺是近年来尝试用于美白和防晒化妆 品中的一种化学物质:1, 2],但对其效果 和作用机制了解甚少。在国内，我们曾应用 原代培养的人皮黑素细胞，探讨了烟酰胺抑 制黑素合成的适宜浓度:3],为进一步明确 烟酰胺对皮肤色素沉着的抑制作用，本文以 棕黄色豚鼠无急性损伤 UVB?黑模型评价烟 酰胺对皮肤色度的影响， 并探讨了可能的作 用机制 1材料与方法 实验动物 体重（355 土 12）g,健康成年雌 性Hartley棕黄色豚鼠10只。 受试物carpobol基质；将烟酰胺溶于 carpobol中分别配成％、 5%、10%三种浓 度。 动物模型 在豚鼠背部选定4cn^ 4cm区域 脱毛后用TL12Rs型UV-B灯管按照/d剂量 照射10天，辐照总量为165mA照射结束后 将产生豚鼠背部皮肤分为 4个相离区域，分 别外涂carpobol基质、％、5%、10%烟酰 胺，每日2次，共计4周。每周用Lab色度 仪测定