CRISPR-Cas9 中文讲解学习.pptVIP

CRISPR-Cas9 中文;背景介绍:; CRISPR-Cas9技术的设计和实现简单，使得世界各地的实验室都可以快速地将其引入生物学研究中。;CRISPR/Cas9 能做什么?;1. 工程细胞和生物模型;CRISPR interference (CRISPRi) KRAB CRISPR activation (CRISPRa) VP64 CRISPR labeling (CRISPR-l) EGFP ;CRISPR/Cas9 system of RNA-guided genome editing is revolutionizing genetics research in a wide spectrum of organisms.;Traditional KO Conditional KO LoxP/loxP-Cre Knockin （敲入） Point mutation （点突变）;3. CRISPR/Cas9基因敲除;4. Cas9与传统基因打靶 时间对比;?Metabolism model;Realtime position of protein！ No good antibody？---Cas9 help！;Conditional Gene Knockout;Recovery or Construction of disease model in cell line