均质式全自动萤光免疫分析仪kryptor.pptVIP

Reagent + Sample 337nm 620nm/665nm Dispense (T 0 ) Detecting Phase (T 1 ), (T 2 ), (T 3 )… Counting Phase (T end ) 337nm 620nm/665nm 337nm 620nm/665nm 檢体濃度在儀器線性範圍內 均質式 全自動螢光免疫分析儀 KRYPTOR KRYPTOR ◆ Homogeneous method in liquid phase ◆ Reaction kinetics ◆ Smart autodilution ability Time Resolved Amplified Cryptate Emission Eu 3+ cryptate (Eu 3+ tribipyridine diamine) N N N N Eu 3+ N N N N 1/2 2/2 RECOVERY OF THE SPECTRA DONOR-ACCEPTOR An Internal Reference XL665 Eu 3+ XL665 Energy Transfer Eu 3+ Eu 3+ Excitation Eu 3+ Eu 3+ Reaction well Specific fluorescence KRYPTOR 能量轉移動態圖 KRYPTOR 能量轉移示意圖 KRYPTOR 螢光產生示意圖 1/5 雷射激發後產生螢光示意圖 2/5 雷射激發後產生螢光示意圖 3/5 雷射激發後產生螢光示意圖 4/5 雷射激發後產生螢光示意圖 5/5 雷射激發後產生螢光示意圖 0 5 1 0 1 5 2 0 0 5 1 0 1 5 2 0 2 5 3 0 4 0 5 0 6 0 2 5 n g /m l 7 0 0 n g /m l 2 ( S e c ) AFP concentration (ng/ml) Dilution factor 0 -700 1/1 ( R F U ) AFP 濃度為 25 及 700ng/ml 之反應曲線 0 5 10 15 20 0 5 10 15 20 25 30 40 50 60 25ng/ml 700ng/ml 5,000ng/ml 2 (Sec) * * * * * * * ? ? ? ? ? ? ? ng/ml 10 6 ng/ml 10 5 * AFP concentration (ng/ml) Dilution f actor 700 -10,000 1/50 ( R F U ) AFP 濃度為 5,000 ng/ml 之反應曲線 0 5 10 15 20 0 5 10 15 20 25 30 40 50 60 25ng/m 700ng/ml 5,000ng/ml 2 (Sec) * * * * * * * ? ? ? ? ? ? ? ng/ml 10 6 ng/ml 10 5 * AFP concentration (ng/ml) Dilution factor 10,000 -500,000 1/2000 ( R F U ) AFP 濃度為 10 5 ng/ml 之反應曲線 0 5 1 0 1 5 2 0 0 5 1 0 1 5 2 0 2 5 3 0 4 0 5 0 6 0 2 5 n g / m l 7 0 0 n g / m l 5 ,0 0 0 n g / m l 2 ( S e c ) * * * * * * * ? ? ? ? ? ? ? n g / m l 1 0 6 n g / m l 1 0 5 * AFP concentration (ng/ml) Dilution factor 500,000 -1,000,000 1/5000 ( R F