蛋白质翻译后修饰及富集方法.pptxVIP

蛋白质翻译后修饰及富集方法;翻译后化学修饰的主要方式：;蛋白质翻译后修饰和富集方法;1.糖基化修饰蛋白的富集;2.甲基化修饰蛋白的富集;3.乙酰化修饰蛋白的富集;4.泛素化修饰蛋白的富集;磷酸化蛋白质组学;;;柠檬酸优化TiO2富集方法;BACKGROUND;IMAC: preference for multiply phosphorylated peptides;Effect of Citric Acid on TiO2 Enrichment;CATSET---Citric acid-assisted Two-step Enrichment with TiO2 ;CATSET---Citric acid-assisted Two-step Enrichment with TiO2 ;CATSET---Citric acid-assisted Two-step Enrichment with TiO2 ;CATSET---Citric acid-assisted Two-step Enrichment with TiO2 ;Comparison of CATSET with DHB/TiO2;Combining SCX with CATSET;Discussion;金属离子IMAC的优化;;;IMAC-HILIC-TiO2组合富集方法;; ;“In comparison, the previous study identified 4146 unique phosphopeptides with Mascot score 22 in their double triplex SILAC experiment consisting of 116 LC MS/MS analyses performed on an LTQ-Orbitrap and an LTQ-FT-ICR instrument. In this study, however, six 15 cm dishes per condition (95% confluent HeLa cells) were used, which will result in ～12 mg of starting material for each condition,30 times more protein than in our setup. This shows the high efficiency of the presented strategy for global phosphoproteomics, which can be applied to a minimum amount of sample. ”;;重复之间差异太大，结果不稳定 重复较少，难以在统计水平上获得可信的差异。;人造塑料抗体富集方法;Plastic antibodies-molecularly imprinted polymers;The MIPs selectively retained pS and pY Fmoc derivates with a preference forthe complementary amino acid side chains, whereas the nonimprinted polymer displayed weak retention of all analytes;Compared with TiO2;Compared with TiO2