化学发光法原理简介.pdf

化学发光法原理基本介绍 Chemiluminescence immunoassay technology (CLIA) has been widely used in the fields of environmental chemistry, molecular biologics and medicine chemistry because of its wide linear range, fast and convenient operation, ease of automation, high accuracy and sensitivity features. 译：化学发光免疫分析技术（ CLIA ）因其检测范围很宽，操作方便 快速，自动化程度高，极高的精密度和灵敏度等特征，已经广泛的应 用到环境化学、药物化学和分子生物制剂等领域。 Chemiluminescence immunoassay analyzer is used to detect the ultra-trace level of substancesin human blood or other body fluids based on the principle of CLIA: at first according to theory of specific binding of antigen and antibody, the paramagnetic solid-phase carrier and sample (antigen) are bound with fluorescent-labeled antibodies (FLA) in the optimum condition, and then after inject H O and NaOH solution, 2 2 oxidation reaction makes the magnetic nanoparticles got in the first step jump to a unstable high-energy excited state and reduction reaction restores the excited state back to a ground state, the excessive energy during the reduction reaction is released in the form of photons; finally use photomultiplier tube to receive the photons and converted to content information of the testing substance in the sample based on the build-in program. 译 :化学发光免疫分析仪是在化学发光免疫分析技术（ CLIA ）的原理 上用来检测人体血液和其他体液中的超微水平的物质， 具体检测原理 如下：1）根据抗原抗体特异性结合的原理， 顺磁固相载体和样本 （抗 原）与荧光标记的抗体（ FLA ）以最合适的方式结合在一起； 2 ）注 入过氧化氢和氢氧化钠溶液后， 氧化反