变温层积过程中华重楼种胚后熟生理生化的变化.docxVIP

[ 摘要 ]通过观察和测定变温层积处理过程中华重楼种 子胚形态发育进程和体内 SOD，POD，CAT ，MDH ，G-6-PDH 5 种酶活性的变化，探究华重楼种子从休眠状态到解除休眠 状态过程的酶调控机制。结果显示，华重楼种胚在经过 40 d 左右的暖温层积（18± 1） C之后，进入快速发育阶段，同 期种子内在代谢活动显著加强，开始进入生理后熟阶段。在 层积第60?80天种子基本完成形态后熟，尔后转入低温层 积（4± 1） C后进一步促进了种子生理后熟。 MDH和 G-6-PDH 活性在种子层积第 40 天后分别发生显著的下降和 上升，通过调控种子呼吸代谢途径由三羧酸途径为主转为磷 酸戊糖途径为主，促进种子解除休眠。 SOD， CAT 活性在整 个层积过程中没有明显波动，但始终维持一定水平，以保证 种子活力。 POD 活性在进入低温层积阶段后显著上升， 可能 参与调控了种子休眠解除的过程。 [ 关键词 ]华重楼 ;种子休眠 ;胚后 熟;SOD;CAT;POD;MDH;G-6-PDH Physiological and biochemical change of Paris seed in after-ripening during variable temperature stratification LI Zhao-ling1 ， TONG Kai1 ， YAN Shen1 ， YANG Hua1， WANG Qiao2 ， TANG Yong-bin1 ， DENG Meng-sheng1 ， TIAN Meng-liang1* ( 1.College of Agronomy ， Sichuan Agricultural University ， Chengdu 611130， China; 2.Yaan Sanjiu Chinese Herbal Medicine Technology Industry Company ， Yaan 625014， China) [Abstract]In order to explore the dormancy physiological and biochemical mechanism of Paris seeds， the seed embryo growth courses， and the dynamic change of 5 enzymes， include SOD ， POD ， CAT ， MDH ，G-6-PDH were measured during variable temperature stratification. The results indicated that Paris seeds embryo grew quickly after 40 d in warm-stratification (18± 1) °C, at the meantime the metabolic activity was significantly strengthened. These facts showed that Paris seeds turned into physiological after-ripening process. After 60-80 d ， the morphological embryo after-ripping process basically completed， and the following cold-stratification (4 ±1)C furthered Paris seed to finish physiological after-ripening. After 40 d ， the activity of MDH decreased while G-6-PDH increased significantly. This showed that the main respiratory pathway of seed changed from TCA to PPP， which benifited breaking seed dormancy. In the whole period of stratification process ， the activity variation of SOD and CAT was insignificantly and the activity of POD was enhanced significantly after shifting the seed in cold stratification process. This showed that