IBS及抑郁症相关肠道菌群.pptx

IBS及抑郁症相关肠道菌群的基因 组学方法及若干研究进展 Research focuses of my lab • Genome and protein sequence analysis and protein structure analysis • Bioinformatics methods of metagenomic analysis associated with human health and environments • Clinical data analysis and medicine informatics Intestinal Microbiota --- “Forgotten” Human Organ  Gut microbes: 1014 cells – cell numbers10 times larger than human; encode gene number 150 times larger than human genome(Baquero,2012).  Influence alterations in host energy balance and immunity(Sanz,2014).  Mysterious:80% gut microbes can’t be cultured in lab (Kellenberger, 2001). 1. Baquero F, Nombela C. Clinical Microbiology and Infection, 2012, 18(s4): 2-4. 2. Sanz Y, Olivares M, Moya-Pérez Á, et al. Pediatric research, 2014. 3. E. Kellenberger. EMBO reports. 2001, 2(1):5–7 Hot Research Field  2008, NIH initiated HMP(Human Microbiome Project).  2008, European Commission initiated MetaHIT (Metagenomics of the Human Intestinal Tract consortium). Publication number related to intestinal microbiota (Sekirov,2009) 1. Qin J, Li R, Raes J, et al. Nature, 2010, 464(7285): 59-65. 2. Nelson K E et al. Science (New York, NY), 2010, 328(5981): 994. 3. Gut Microbiota in Health and Disease Gut Microbiota and Host Disease  Relationship:  a). Dysbiosis triggers pathogenesis.  b). Dysbiosis arises in parallel with pathogenesis.  c). Disease causes shift in gut flora structure.  d). Dysbiosis aggravates disease. Significance of Gut Flora Dysbiosis(Frank, 2011) 1. Frank D N, Zhu W, Sartor R B, et al. Investigating the biological and clinical significance of human dysbioses[J]. Trends in microbiology, 2011, 19(9): 427-434. Gut Microbiota and Host Disease Gut Microbes and Diabetes (Sanz ,2014) Gut Microbes and Obesity Metabolic Syndrome (Devaraj,2013) 1. Sanz Y, Olivares M, Moya-Pérez Á, et al. Understanding the role of gut microbiome in metabolic disease risk[J]. Pediatric research, 2014. 2. Devaraj S, Hemarajata P, Versalovic J. The human gut microbiome