大鼠pdgf―c基因真核表达载体的构建与鉴定.docxVIP

大鼠PDG— C基因真核表达载体的构建与鉴定 ：Objective To construct and identify a eukaryotic expression plasmid of rat PDGF-C gene. Methods An rat PDGF-C gene fragment was amplified and sequenced ， then cloned into pEGFP-N1 to construct the eukaryotic expression plasmid pEGFP-N1-PDGF-C. The recombinant plasmid was identified by using double restriction enzyme digestion. Results The RT-PCR product was a gene fragment with 1053bp ， and the nucleotide sequence of that was consistent with the sequence of the rat PDGF-C gene registered in GenBank. The recombinant eukaryotic expression plasmid pEGFP-N1-PDGF-C was ident