心脏缺血再灌注损伤研究论文.docxVIP

心脏缺血再灌注损伤研究论文 【关 键 词】 再 灌 注 损 伤 Cha ngesofnitricoxidesyn thesisi ncardiacischemiareperfusi onin jury 【 Abstract ] AIM:Toi nv estigatetheexpressi onandfun ctio nofen dothelial nitri coxides yn thase(eNOS)a ndin ducibleNOS(iNOS)duri ngischemiarepe rfusio ninjury(IRI)i nrathearts.METHODS:IRIwasi nducedbyclampi n gthelefta nteriordesce ndi ng(LAD)ofcoro naryarteryfor1h,follo wedbyreperfusio n.Nitricoxides yn thase(NOS)activitiesofhearts wasassayedatvarioustimepo in tsa ndNOSprote ini evelsaswellasNOS mRNAexpressi on weredetermi nedbyWesternblota ndRTPCRmethodsres pectively.RESULTS:eNOSactivity,pro