海洋生物学课件：Some important types of phytoplankton.pptVIP

Methods Take bottle with phytoplankton, Filter using vacuum-filtration apparatus Place filter in tube, add methanol, agitate and crush filter with metal spatula Put in freezer (-20oC) for 5 minutes Centrifuge vial at top speed (5 minutes) Transfer supernatant to cuvette (~ 2 ml) Put cuvette in fluorometer, read fluorescence Convert to chlorophyll a (fluorometer does this) Lab Report Write up two experiments: How light and nutrients affect phytoplankton production (of oxygen, of cells/biomass) concentration versus chlorophyll a Remember the big picture/broader impact for discussion/introduction: how light and nutrients affect phytoplankton production in the ocean, what about nutrient limitation, light limitation changes in both over time, with nutrients in proximity to coastal zone (humans) Cloudy versus sunny days 复习思考题 解释：水华，赤潮，囊壳，蛋白核。 何谓藻类？藻类包括那些类群？ 藻类的色素组成的特点是什么？ 简述各门藻类同化产物的类型、特点和加碘染色后特征。 简述藻类生活史类型及其特点。 试述主要藻类类群在海洋中的生态功能与环境的关系。 * Some important types of phytoplankton Diatoms: Phylum Chrysophyta Dominant in temperate and polar waters Silica case or shell looks like a pill box“; Found singly or in chains; Planktonic forms are radially symmetrical Can reproduce very quickly, up to 6x/day via asexual reproduction (also have sexual reproduction) Dinoflagellates Dominant in tropical and subtropical waters also summer in temperate areas they have two flagella and a shell of cellulose plates (called theca) Asexual reproduction and fast population growth can lead to red tides They secrete a neurotoxin called saxitoxin: bioaccumulates in shell fish and other filter feeders... can be fatal Coccolithophores Tropical... often very common Calcium carbonate shells or tests Their skeletons make important depositional structures, but naked forms are not preserved Silicoflagellates: Biflagellated, silica internal skeleton... found world wide, particularly in Antarctic Cyanobacteria: Blue-green algae but not true algae: often in brackish nearshore waters and warm water gyres the