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硕 士
硕 士 学 位 论 文 英 文 摘 要
A B S T R A C T
B a e吨 ro u n d
Th e g lu eo se一regu lated P rotein s (G 砂 ) refer to a set o f P rotein s g en erate d b y ee ll in o rd er to ad aPt to en d op la sm ie re tieu lu m stre s s .G R P 7 8 an d G R P 9 4 ar e imP o rtant m e mb e rs o f th e tri b e ,w h o se m a in P 坤 sio log ieal fun etion 15 in v o lv ed in P rotein syn th esis wh en th e n ewb om
P o lyP ePt id e eh ain fo ld in g ,a s m o le eu lar eh aP ero n e s.Re cent stu d ie s sh o w
that,h igh exP ression o f G RP w as fou n d in som e tum or eells,they w ere
inv o lv e d e lo se ly in th e g e n e ratio n , P ro gr e s sio n an d P ro g n o sis o f m a lign ant tum o rs ,an d suP P ly n e w th e o ry suP P o rt fo r e lin ic a l d ia gn o s is an d tre atm e ni fo r m a lign an t tu m o rs .
O bj eetiv e
To stu 即 the eXP ression s o f G RP 7 8 an d G R P 9 4 in reetal a d e n o e ar e in o m a an d th e re lat io n sh iP b e tw e e n th e m an d th e e lin ie a l an d P ath o lo g ie a l fe atu re s o f re c ta l ad e n o c ar e ino m a ,an d its e lin ie al s ign ifie an c e ·
M e th o d s
We C o lle e te d 4 5 e a s e s o f su rg ie a lly re s e e te d sP e e im e n s in re c ta l
a d e n o e ar e in o m a an d th e ir P e ric an e e ro u s tis su e s fo r e o ntr a st. S P e e im e n s w e re e o n firm e d b y P ath o lo gy ex am an d e lin ie a l d ata ini e gr ity. A ll th e sP e e im e n s w e re fix e d in 1 0 % fo rm a lin an d ro ut in e ly e m b ad d e d in p araffi n .E 而 sionTM w as u sed 允r ass叮 in g 小e e即 ressio n s o r o 即 7 5 an d
G 即 9 4 ,an d seored them u n d er h igh 一P o w er m iero sco P y .P u t the d ata into
S P S S 13 .0 an d an a ly z e th e e o r
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