基因的分子生物学dnareplication.pptxVIP

14 DNA REPLICATION2007-04-274.14.1 IntroductionThe replisome is the multiprotein structure that assembles at the bacterial replicating fork to undertake synthesis of DNA. It contains DNA polymerase and other enzymes.A dna mutant of bacteria is temperature-sensitive; it cannot synthesize DNA at42°C, but can do so at 37°C.A quick-stop mutant is a type of DNA replication temperature-sensitive mutant(dna )in E. coli that immediately stops DNA replication when the temperature isincreased to 42°C.A slow-stop mutant is a type of DNA replication temperature-sensitive mutant in E. coli that can finish