干细胞移植治疗AMI临床研究进展教学PPT课件.ppt

Neovascularization Neovascularization induced by intracoronary infusion of BMC may be a key mechanism leading to recovery of contractile function and subsequent reduction of clinical event rate. 心脏功能及预后改善机制 A substudy of REPAIR-AMI assessing the effect of intracoronary BMC administration on coronary flow dynamics using intracoronary Doppler flow velocity measurements at baseline and at 4 month follow-up: Significant greater recovery of coronary blood flow reserve (CFR) in the BMC-treated infarct artery compared with infarct vessels receiving placebo infusion. Circulation. 2019; 116:366–374 Paracrine effects Various studies confirmed that progenitor cells release paracrine factors (cytokines and growth factors) that modulate angiogenesis, cardiomyocyte apoptosis, fibrosis, and inflammation. Fibrogenesis Tissue Repair. 2019 Oct 13;1(1):4 J Cardiovasc Transl Res. 2019 Feb 26. [Epub ahead of print] Swine subjected to AMI by temporary balloon occlusion of the LAD using percutaneous techniques received intracoronary injection of either concentrated MSC-derived growth factors or control medium. MSC-derived factors significantly reduced cardiac troponin-T elevation and improved echocardiographic parameters, decreased the fibrotic area, reduced myocardial damage and prevented cardiomyocyte apoptosis. 旁分泌因子作用 Strategies designed to augment MSC paracrine function have been employed in an attempt to improve their therapeutic efficacy. It has been demonstrated that treating MSCs with transforming growth factor-α (TGF-α) can stimulate VEGF production in vitro. 扩大旁分泌作用 Am J Physiol Regul Integr Comp Physiol. 2019;299(1):R371-8 Using a model of isolated heart perfusion, MSCs pretreated with TGF-α was associated with decreased myocardial injury and increased myocardial function after global ischemia/ reperfusion when compared to infusion of untreated MSCs. Circulation. 2019;121:2019-2019 Methods and Results: Vectors that encoded inducible suicide genes unde