重组人生长激素作用下成骨细胞细胞周期及细胞骨架改变的体外研究.pdfVIP

重组人生长激素作用下成骨细胞细胞周期及细胞骨架改变的体 外研究 成骨细胞；重组人生长激素；细胞周期 ;细胞骨架 【Abstract 】 AIM: To observe the changes of the cell cycle and cytoskeleton of rat calvarial osteoblasts after stimulated by recombinant human growth hormone (rhgh ). METHODS: Osteoblastic cells from rats were incubated in the medium supplemented wiht rhgh at different doses (10, 200 μg/L), and then the changes of cytoskeleton and cell cycle were detected. RESULTS: Quantitative analysis showed that osteoblasts were arrested in S phase and G2/M phase, however, green fluorescent density and red f