医学交流课件：Biomarkers and phenotyping in asthma.pptxVIP

Biomarkers and phenotyping in asthma;Unbiased Biomarkers for the Prediction of Respiratory Disease Outcomes ;University of Amsterdam, University of Southampton, Imperial College London, University of Manchester, University of Nottingham, Fraunhofer Institute Hannover, CNRS-EISBM Lyon, Université de Méditerranee Montpellier, Karolinska Institute Stockholm, University Hospital Umea, University Tor Vergata Rome, Università Cattolica del Sacro Cuore Rome, University of Catania, Hvidore Hospital Copenhagen, University Hospital Copenhagen, Haukeland University Bergen, Semmelweis University Budapest, Jagiellonian University Krakow, University Hospital Bern, University of Ghent ;;;Th2-high vs Th2-low in mild-moderate asthma;How common is a Th2 (IL-13) high in severe asthma? ;(11%);101 patients (32%) were low for all 3 biomarkers;How to approach phenotyping/endotyping/clustering/fingerprint/handprint?;PARAMETERS USED -Age of asthma onset - Pack-years of smoking - Body Mass Index (BMI) - FEV1 % predicted - FEV1/FVC - Asthma Control Questionnaire: ACQ-5 - Exacerbations in past year -Medication Oral Corticosteroid (OCS) daily dose ;UBIOPRED asthma clusters based on unsupervised clustering of 8 clinical features;UBIOPRED : smoking (cluster 2) vs non-smoking (cluster 3) of airflow obstruction;Wheelock et el. ERJ 2013;42:802;Analysis of sputum inflammatory cells transcriptomics and proteome: ‘sputum fingerprint’;Distribution of neutrophilic and eosinophilic inflammation in sputum;IL-1 receptor associated gene expression;FC 1.62, FDR 0.007;Hierarchical Clustering;;;Receptor: TREML2, CLEC4D,IFIH1,SIGLEC5,LILRA5;Fatty acid metabolism: ME1 ;Expression of various gene signatures in the 3 TACs using gene set variation analysis;?;; Top 10 enrichment from public ontology databases of the 284 differentially-expressed genes;U-BIOPRED Sputum Handprint of Severe Asthma;Platforms used for Sputum ‘omics analyses;Data Integration;;;;Impaired host defence;;;Conclusions;Thanks to…