儿童肺炎支原体感染三种实验诊断方法评价（基础医学范文）.docVIP

儿童肺炎支原体感染三种实验诊断方法评价（基础医学范文） 文档信息 属性： F-00P9FZ，doc格式，正文7535字。质优实惠，欢迎下载！ 适用： 作为文章写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 作者： 佚名 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 搞要 1 关键字：肺炎支原体； 抗体检测； 抗原检测； 荧光定量PCR 2 1 材料与方法 4 2 结果 5 3 讨论 6 参考文献 8 论文原创声明（模板） 11 论文致谢（模板） 11 正文 儿童肺炎支原体感染三种实验诊断方法评价（基础医学范文） 搞要 摘要：目的：探讨儿童肺炎支原体感染的快速、简便、有效的临床实验室诊断方法。方法：对2016年12月收集的200例临床呼吸道感染患儿的咽拭子和静脉血样本进行肺炎支原体快速鉴定培养镜检、抗体检测、抗原检测及荧光定量PCR（Polymerasechainreaction）分析。以快速培养鉴定镜检法为诊断肺炎支原体感染金标准，比较抗体检测、抗原检测及荧光定量PCR三种诊断方法的灵敏度、特异度和Youden指数（正确诊断指数）。结果：抗体?z测的灵敏度、特异度和Youden指数分别为%、%、%；抗原检测为%、%、%；荧光定量PCR为%、%、%。荧光定量PCR法的灵敏度、特异度和Youden指数均高于抗体检测和抗原检测法（P），三者Youden指数两两比较，差异均有统计学意义（P）。结论：荧光定量PCR具有较高的灵敏度、特异度和Youden指数，可以在未来替代快速鉴定培养镜检、抗体检测和抗原检测法，作为临床诊断儿童肺炎支原体感染的快速、简便而有效的实验室诊断方法 关键字：肺炎支原体； 抗体检测； 抗原检测； 荧光定量PCR 【Abstract】 Objective：To explore the fast，simple and effective method to clinical diagnosis of mycoplasma pneumonia infection of ：Throat swabs and venous blood samples were obtained from 200 children with respiratory infection，these samples were tested by rapid identification culture and microscopy，antibody detection，antigen detection，fluorescence quantitative PCR in December identification culture and microscopy was set as the diagnostic “gold standard”，sensitivity，specificity and youden index of three methods were ：The sensitivity，specificity and youden index of antibody detection were respectively %，% and %；antigen detection were respectively %，% and %；fluorescence quantitative PCR were respectively %，% and %.The sensitivity，specificity and youden index of fluorescence quantitative PCR were higher than those of antibody detection and antigen detection（P），compared youden index of three methods，the differences were statistically significant（P）.Conclusion：The sensitivity，specificity and Youden index of fluorescence quantitative PCR are higher，it can take the place of rapid identification culture and microscopy，antibody detection and antigen detection in the future，and it is a rapid，convenient and ef