系统性红斑狼疮皮损角质形成细胞体外培养及细胞学特性观察（临床医学论文）.docVIP

系统性红斑狼疮皮损角质形成细胞体外培养及细胞学特性观察（临床医学论文） 文档信息 主题： 关于“医学心理学”中“皮肤病学与性病学”的参考范文。 属性： F-00BP20，doc格式，正文6886字。质优实惠，欢迎下载！ 适用： 作为医学论文、临床医学写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 作者： lunwenchina 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 搞要 2 关键字：SLE; 角质形成细胞; CCK-8; 凋亡; 细胞周期 2 1 材料和方法 3 2 结 果 6 3 讨 论 7 【参考文献】 9 论文原创声明（模板） 10 论文致谢（模板） 10 正文 系统性红斑狼疮皮损角质形成细胞体外培养及细胞学特性观察（临床医学论文） 作者：赖宽 曾凡钦 魏菁 郭庆 何嘉辉 谭国珍 李伯有 搞要 摘要：目的体外培养系统性红斑狼疮(SLE)皮损部位角质形成细胞(KC)，观察其细胞学特性。方法原代培养SLE皮损和正常皮肤的角质形成细胞，倒置显微镜观察细胞的形态特点，免疫荧光法进行角蛋白检测并计算细胞纯度;两步消化法纯化细胞;CCK-8(cellcountingKit-8)评价细胞的增殖情况，绘制生长曲线。流式细胞仪观察细胞的生长周期和凋亡情况。结果使用K-SFM(serum-freekeratinocytemedium)无血清培养基能成功体外培养SLE皮损的角质形成细胞，呈铺路石样。结合人工纯化，能达到95%的纯度;与正常对照相比，SLE皮损角质形成细胞进入指数生长期较迟(7dvs.4d)，但进入后增长迅速，S期比例为：(±)%vs.(±)%(P);凋亡率高：(±)%vs.(±)%(P)。结论SLE皮损角质形成细胞培养难度大，细胞的生物学行为存在异常，可能为SLE发病的独立、始发因素，其致病机制值得深入研究和探讨。英语论文发表 关键字：SLE; 角质形成细胞; CCK-8; 凋亡; 细胞周期 Abstract： 【Objective】 To observe the cytologic characteristics of keratinocytes (KC) from lesion of systemic lupus erythematosus (SLE) patients in vitro. 【Methods】 KC from lesions of SLE patients and healthy people (control group) were cultured in vitro and identified by detecting Pan cytokeratin with immunofluorescence. Features of the keratinocytes were analyzed with invert microscope. Two-step digestion when passaging was used to purify cell line. CCK-8 was used to assay growth kinetics of the keratinocytes and come up with a growing curve after continuous analysis for two weeks. Cell circle and apoptosis were observed by flow cytometry. 【Results】 KC was cultured in vitro successfully. Concerning passage 2， over 95% of the cells from primary culture were positive for cytokeratin. Comparing with KC from healthy people， KC from SLE lesions needed more time (7 d vs. 4 d) to reach exponential growth phase (EGP)， but grew faster in EGP. Both cell count at S phase and apoptosis rate of KC from SLE lesions elevated， when comparing with that of the control group. 【Conclusion】 Keratinocytes from lesions of SLE patien