高效液相色谱法测定三棱超临界萃取物中芦丁的含量.pdfVIP

如文档对您有帮助，欢迎下载支持，谢谢！ 高效液相色谱法测定三棱超临界萃取物中芦 丁的含量 【摘要】 目的 建立三棱超临界 CO2萃取物中芦丁的鉴别和 含量测定方法。方法在聚酰胺膜上点样，以 80%乙醇作为展开剂鉴别 芦丁，采用 Symmetry C18色谱柱，以乙腈 - 四氢呋喃 -0.2%枸橼酸 （14 ∶ 1 ∶85）为流动相，柱温 25℃，在流速为 1.0 ml ·min-1 ，360 nm 的 检测波长下，测定芦丁含量。结果在三棱的超临界萃取物中含有芦丁 成分，建立的液相色谱方法在芦丁浓度为 0.003 40～0.02432 mg·ml-1 的范围内线性关系良好，样品在 8 h 内稳定，平均加样回收率为 98.56%，以峰面积作精密度实验， RSD为 1.38%。结论三棱的超临界萃 取物中含有芦丁，建立的高效液相色谱方法线性关系良好，精密度和 回收率高，适合于三棱中芦丁含量的测定。 【关键词】 反相高效液相色谱； 薄层色谱鉴别； 芦丁； 超临 界萃取 Abstract:ObjectiveTo explore whether there is rutin in SFE extract of Sparganium stoloniferum by TLC and establish a method to determine its content by RP-HPLC. Methods The determination was carried out over a SymmetryC18column at 25 ℃. Methods tetrahydrofuran: 0.2% citric acid (14 ∶1 ∶85) was used as mobile phase at a velocity of 1 ml ·min-1 and the detection wavelength was set at 360nm. ResultsRutin was found in SFE extract of Sparganium Stoloniferum. The calibration curve was 如文档对您有帮助，欢迎下载支持，谢谢！ linear in the range of 0.003 40～0.02432 mg·ml-1 for rutin. The sample was stable within 8 hours and the recovery rate of rutin was 98.56 %. And in precision test, RSD=1.38%.ConclusionThere is rutin in SFE extract of Sparganium stoloniferum. The established RP-HPLC method has perfect linear relationship, high precision and recovery rate and it is suitable to determine the content of rutin in SFEextract of Sparganium Stoloniferum. Key words: RP-HPLC; TLC; Rutin; Supercritical extract 三棱 Sparganium stoloniferum ，始载于《本草拾遗》 ，为常 用中药，是荆三棱科植物黑三棱的干燥