MEEKC-LVSS法测定五种黄酮类化合物.docVIP

PAGE PAGE 24 MEEKC-LVSS法测定五种黄酮类化合物 中文摘要 目的将CE新分离模式MEEKC与LVSS结合以提高检测灵敏度及减小生物样品基质的干扰，并利用MEEKC-LVSS法分离检测了表儿茶素、槲皮苷、山奈酚、木犀草素、槲皮素等5种黄酮类化合物，并将所建立的新方法用于人加标尿样和野菊花提取物中黄酮类化合物的检测。 将CE新分离模式MEEKC与LVSS结合以提高检测灵敏度及减小生物样品基质的干扰，并利用MEEKC-LVSS法分离检测了表儿茶素、槲皮苷、槲皮素、木犀草素、山奈酚等5种黄酮类化合物，并将所建立的新方法用于人加标尿样和野菊花提取物中黄酮类化合物的检测。 方法实验条件：缓冲液：0.3%（w/v）；十二烷基硫酸钠SDS, 1%（v/v）；乙酸乙酯, 2%（v/v）正丁醇；97%（v/v）20 mM硼砂- 40 mM 磷酸二氢钠（pH 9.0），进样3.0 psi × 55 s，堆积时间20 kV×1.25 min。 压力进样0.5 psi × 6 s; 检测波长214 nm; 运行电压20 kV。 结果表儿茶素、槲皮苷、山奈酚、木犀草素、槲皮素浓度分别在0.05-2μg/mL( r = 0.9984)、0.02-2μg/mL( r = 0.9994)、0.02-1μg/mL( r = 0.9995)、0.02-1μg/mL( r = 0.9929)、0.02-1μg/mL( r = 0.9996)线性关系良好，平均加样回收率分别为表儿茶素、山奈酚、木犀草素、槲皮素108.39%、99.67%、95.23%、104.92%；RSD分别为0.74、6.18、5.35、2.76。 结论该方法不仅提取效率高,分离效果好,运行费用低,重现性高，大体积进样增大了对于微含量的黄酮类化合物的检测。 关键词微乳液毛细管电动色谱大体积进样堆积黄酮类 Abstract Objective The new CE separation mode MEEKC and lvss combined to improve the detection sensitivity and reduce the interference of the matrix of biological samples, and the MEEKC-LVSS separation method to detect the epicatechin, quercetin, kaempferol, luteolin and quercetin five flavonoid compounds, and established a new method for the detection of flavonoid compounds in spiked human urine and wild chrysanthemum extract. Be a combination of new CE separation mode MEEKC and lvss to improve the detection sensitivity and reduce the interference of the matrix of biological samples, and the MEEKC-LVSS method for separation and detection the epicatechin, quercetin, quercitrin, wood Rhino grass, kaempferol 5 kinds of flavonoids compounds and established the new method for the detection of flavonoid compounds in spiked human urine and wild chrysanthemum extract. Methods Experimental conditions: buffer: 0.3% (w / V); sodium dodecyl sulfate (SDS), 1% (V / V); ethyl acetate, butanol 2% (V / V); 97% (V / V) 20 mm borate - 40 mM sodium dihydrogen phosphate (pH 9.0), 3.0 psi x 55 s, the accumulation time of 20 kV x 1.25 min. pressure injection 0.5 psi x 6 s; detection wavelength was 214 nm; ope