宏基因组高通量测序技术应用于感染性疾病病原检测中国专家共识.pdfVIP

中华检验医学杂志2021年2月第44卷第2期 ChinJLabMed,February2021,Vol.44,No.2 ·107 · ·指南与共识· 宏基因组高通量测序技术应用于感染性疾 病病原检测中国专家共识 中华医学会检验医学分会临床微生物学组中华医学会微生物学与免疫学分会临床微 生物学组 中国医疗保健国际交流促进会临床微生物与感染分会 通信作者：王辉，Email：whuibj 163.com 【摘要】 宏基因组高通量测序技术通过对临床样本中微生物和宿主核酸的测序分析，可以无偏 倚地检测多种病原微生物，正在逐渐应用于临床感染性疾病病原检测，然而业界对该技术的临床适应 证、实验流程、质量管理、性能验证和报告解读等方面仍有困惑。中华医学会检验医学分会临床微生 物学组、中华医学会微生物学与免疫学分会临床微生物学组、中国医疗保健国际交流促进会临床微生 物与感染分会组织专家对上述问题进行了讨论并撰写了专家共识，对一些关键问题给出了推荐意见 和处理方法，希望有益于业界的良性互动，促进该技术规范和发展，为临床抗感染诊治提供帮助。 【关键词】 宏基因组； 二代测序； 感染性疾病； 病原检测 基金项目：国家重点研发计划（2018YFE0102100）；国家自然科学基金 Chinese expert consensus on metagenomics next‐generation sequencing application on pathogen detection of infectious diseases Clinical Microbiology Group of Chinese Society of Laboratory Medicine, Clinical Microbiology Group of Chinese Society of Microbiology and Immunology, Society of Clinical Microbiology and Infection of China International Exchange and Promotion Association for Medical and Healthcare Corresponding author: Wang Hui, Email: whuibj@163.com. 【Abstract 】Metagenomic next‐generation sequencing, the analysis of microbial and host genetic material in samples from patients, can detect pathogens unbiasedly. The emerging approach is rapidly moving to clinical laboratories for pathogen detection in infectious diseases. However, there are some problems about the clinical indications, experimental procedures, quality management, performance verification and report interpretation of the technology. Experts from the Clinical Microbiology Group of Chinese Society of Laboratory Medicine, the Clinical Microbiology Group of Chinese Society of Microbiology and Immunology, and the Society of Clinical Microbiology and Infection of China International Exchange and Promotion Association for Medical and Healthcare discussed the a