荧光显微镜的基本使用方法.pptVIP

??细胞生物学实验 ;实验目的;自发性荧光物质;优点： 检出能力高 对细胞的刺激小 能进行多重染色 用途： 物体构造的观察 荧光的有无、色调比较进行物质判别 发荧光量的测定对物质定性、定量分析;实验原理;实验原理;Microscopes with an upright-style frame are capable of producing fluorescence illumination either through episcopic or diascopic optical pathways, although the latter is rarely used today. Epi-illuminators usually consist of a mercury or xenon lamphouse coupled to a vertical illuminator that is positioned above the main frame in a separate assembly. The microscope nosepiece and transmitted light components (diascopic illuminator, condenser, field diaphragm, filters, etc.) are built into the main frame, while fluorescence components are housed in the vertical illuminator. These illuminators often contain a revolving or sliding turret that houses four to six cubes that contain a mixture of interference filters including a barrier filter, dichroic mirror, and an excitation filter. As illustrated above, light emitted from the lamp positioned in the episcopic lamphouse passes through a collector lens and then the field and aperture diaphragms before entering the first interference filter in the cube set, the emission filter. This light is then directed through the objective and onto the specimen by a special dichroic mirror that reflects certain wavelengths while passing others. Secondary fluorescence, emitted by fluorophores residing in the specimen, travels back through the objective and the dichroic mirror before passing through a barrier filter and into the microscope eyepieces or camera system.;激发光源;激发滤色块;多用途型BX-RFA(适合多种科研要求)及通用型BX-URA2。可同时使用6种荧光激发块， “轻拨式”激发块转换功能，使不同荧光激发之间的迅速转换非常方便，并可消除震动，有利于复染样品的荧光观察。激发块标识在暗室环境中发光，在暗室中也能很容易地选择需要的荧光激发块。另外，根据需要还可以选择6孔滤色片滑块(U-RSL6/U-RSL6EM)安放激发滤色片和发射滤色片进行荧光观察。 ;BX51 在镜基上装有3个滤色片（ND6，ND25，LBD），另外，还可以选装第四个。镜基侧面的拉杆可以很容易地插入/取出滤色镜。;1、?该镜可作为明视场显微镜使用，也可作为荧光显微镜使用，根据需要，做相应的附件连接，开启相应的电源。禁止乱调设置，以免影响他人正常使用。 2、?作为明视场显微镜使用；A、接通电源后，打开显微镜底座前的电源开关，按光亮需要调节底座右侧的滑竿。B、根据需要，调节底座光栅和聚光器光栅及其高度，可获得最佳的图象效果。C、需要图片，要把镜体上的相应拉杆拉到绿线位子。按下相应附件上的照相按扭。 3、?作为荧光显微镜