ATP检测试剂盒产品使用说明书.pdf

Datasheet 爱必信（上海）生物科技有限公司 货号：abs580117 品名：ATP Microplate Assay Kit 规格：96T ATP Microplate Assay Kit User Manual Detection and Quantification of ATP content in Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples. For research use only. Not for diagnostic or therapeutic procedures. Datasheet 爱必信（上海）生物科技有限公司 I. INTRODUCTION2 II. MATERIALS REQUIRED BUT NOT PROVIDED 2 III. KIT COMPONENTS3 IV. SAMPLE PREPARATION 4 V. ASSAY PROCEDURE 5 VI. CALCULATION 6 VII. TYPICAL DATA 7 VIII. TECHNICAL SUPPORT 7 IX. NOTES 7 1 Datasheet 爱必信（上海）生物科技有限公司 I. INTRODUCTION ATP (Adenosine 5-triphosphate) is the chemical energy for cellular metabolism and is often referred to as “energy currency of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. ATP Microplate Assay Kit is a sensitive assay for determining ATP in various samples. ATP concentration is determined by creatine kinase and creatine. The reaction products can be measured at a colorimetric readout at 635 nm. II. MATERIALS REQUIRED BUT NOT PROVIDED 1. Microplate reader to read absorbance at 635 nm 2. Distilled water 3. Pipettor, multi-channel pipettor 4. Pipette tips 5. Mortar 6. Centrifuge 7. Timer 8. Ice 2 Datasheet 爱必信（上海）生物科技有限公司 III. KIT COMPONENTS