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Datasheet 爱必信（上海）生物科技有限公司 货号：abs580131 品名：6-Phosphogluconate Dehydrogenase Microplate Assay Kit 规格：96T 6-Phosphogluconate Dehydrogenase Microplate Assay Kit User Manual Detection and Quantification of 6-Phosphogluconate Dehydrogenase Activity in Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples. For research use only. Not for diagnostic or therapeutic procedures. Datasheet 爱必信（上海）生物科技有限公司 I. INTRODUCTION 2 II. KIT COMPONENTS3 III. MATERIALS REQUIRED BUT NOT PROVIDED 3 IV. SAMPLE PREPARATION 4 V. ASSAY PROCEDURE5 VI. CALCULATION 6 VII. TYPICAL DATA 7 VIII. TECHNICAL SUPPORT 7 IX. NOTES 7 1 Datasheet 爱必信（上海）生物科技有限公司 I. INTRODUCTION 6-Phosphogluconate dehydrogenase (6PGD) is an enzyme in the pentose phosphate pathway. It forms ribulose 5-phosphate from 6-phosphogluconate. It is an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways (PPP). Prokaryotic and eukaryotic 6PGD are proteins of about 470 amino acids whose sequences are highly conserved. The protein is a homodimer in which the monomers act independently: each contains a large, mainly alpha-helical domain and a smaller beta-alpha-beta domain, containing a mixed parallel and anti-parallel 6-stranded beta sheet. NADP is bound in a cleft in the small domain, the substrate binding in an adjacent pocket. The reaction velocity is determined by measuring the increase in absorbance at 340 nm resulting from NADPH.