十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响.docVIP

十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响 1 1 材料与方法 2 2 结果 4 3 讨论 6 文2：睡眠剥夺对大鼠神经内分泌功能变化的影响 7 1材料与方法 8 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响 文1：十一方药酒对大鼠皮肤深Ⅱ度烫伤创面愈合的影响 Abstract：ObjectiveTo observe the effects of Shi Yi Fang vinum on deep partial thickness burn wound in A model of deep partial thickness burn wound in rats was used. All wounded rats were divided into five groups, different concentration of Shi Yi Fang vinum was used, the wound healing time , the content of hydroxyproline, the rates of wound healing as well as the histopathological change of the wound tissues were examined at different time points post burn, so as to value the effects of Shi Yi Fang vinum on the healing of the deep partial thickness burn wounds of rats. ResultsCompared with negative control group, the healing time was shorter and the ratio of wound healing was higher, the content of hydroxyproline was obviously higher in wounds of the Shi Yi Fang vinum group. The histopathological examination also indicated that the proliferation conditio of epithelium and fibroblast cells were much better in the Shi Yi Fang vinum group. Conclusion It is suggested that external application of Shi Yi Fang vinum to deep partial thickness burn wound in rats may stimulate collagen synthesis and enhance wound healing. Key words：Shi Yi Fang vinum; Burn; Wound healing; Hydroxyproline 十一方药酒主要由乳香、没药、红花、 自然 铜、续断等中药制成。该药酒具有活血祛淤、消肿止痛、续筋接骨、止血生肌等作用。临床上常用于跌打损伤， 治疗 各种外伤肿痛、骨折、腰腿痛。疗效明显。现临床上有试用于治疗烧、烫伤，并且效果良好。本实验在大鼠皮肤深Ⅱ度烫伤创面外用该药，观察不同剂量十一方药酒对烫伤创面愈合的影响，为临床用药提供依据。 1 材料与方法 药品、试剂与仪器十一方药酒（广西中医学院第一附属 医院 制剂室，批号）湿润烧伤膏(汕头 经济 特区美宝制药厂, 批号)。考马斯亮蓝及羟脯氨酸测试盒(南京建成生物工程研究所) 。722 分光光度计(上海分析仪器总厂) , 电热恒温水浴箱(重庆实验设备厂) 动物模型制备及分组 选用健康的Wistar系白色大鼠，体重200～250g，雌雄各半，(广西药品检验所实验动物中心提供，许可证号 SCXK 桂，) 用10％水合氯醛（300 mg/kg）腹腔注射麻醉, 背部脱毛, 次日同法麻醉, 背部置于80 ℃ 水中烫15 s, 面积约10% 总体表面积(TBSA ), 造成深Ⅱ度烫伤模型(经病理证实)［1］。烫伤后立即腹腔注射乳酸林格氏液5ml。造模大鼠醒后分笼饲养, 自由饮水进食。动物随机分为5组，每组各30只大鼠。I组生理盐水阴性对