银杏叶提取物诱导血红素氧合酶1表达抗缺血再灌注损伤后心肌细胞凋亡的研究.docVIP

银杏叶提取物诱导血红素氧合酶1表达抗缺血再灌注损伤后心肌细胞凋亡的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：银杏叶提取物诱导血红素氧合酶1表达抗缺血再灌注损伤后心肌细胞凋亡的研究 1 1 材料与方法 3 2 结果 5 3 讨论 6 文2：银杏叶提取物诱导大鼠主动脉平滑肌细胞中血红素氧合酶1表达的实 7 1 材料与方法 8 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 12 正文 银杏叶提取物诱导血红素氧合酶1表达抗缺血再灌注损伤后心肌细胞凋亡的研究 文1：银杏叶提取物诱导血红素氧合酶1表达抗缺血再灌注损伤后心肌细胞凋亡的研究 Abstract：ObjectiveTo explore the protective effects and mechanism of heme oxygenase-1 (HO-1) expression induced by extract of Ginkgo biloba(EGb761) agait cardiomyocyte apoptosis from ischemia reperfusion injury(IRI).Methods48 rabbits were randomly divided into simple IR group, EGb761 group and EGb761+ ZnPPⅨ group. Each rabbit in different groups was intraperitoneal injected with saline(5ml), EGb761(10mg/kg), EGb761(10mg/kg)+ ZnPPⅨ(/kg) respectively. 24 hou later, 8 rabbits in each group were killed to measure myocardial HO-1 expression and HO-1 activity. Other rabbits were subjected for 40min of myocardial ischemia by ligation of left anterior descending coronary artery followed by 4h of reperfusion. The neutrophil infiltration, myocardial malonaldehyde(MDA) content, Bax and Bcl-2 protein expression, cardiomyocyte apoptosis index(AI) in ischemia areas were with simple IRI group and EGb761+ ZnPPⅨ group, the expression and activity of myocardial HO-1 increased significantly, the neutrophil infiltration and MDA content decreased. Bax expression increased and AI and Bcl-2 level decreased in EGb761 group(P respectively). Compared with simple IRI group, EGb761+ ZnPPⅨ group showed higher myocardial HO-1 expression but lower HO-1 activity (P), while the MDA content increased (P),but the neutrophil infiltration, Bax and Bcl-2 expression and AI showed no significant could induce HO-1 overexpression, through its antiinflammatory and antioxidative action to upregulate Bcl-2 and downregulate Bax, thus protect agait cardiomyocyte apoptosis from IRI. Key words：Extract of Ginkgo biloba; Heme oxygenase-1; Reprfusion injury; Apoptosis 银杏叶提取物(EGb761)被广泛用于缺血性心、脑血管病