羊胎素对体外骨髓基质细胞的增殖和向成骨细胞分化的影响.docVIP

羊胎素对体外骨髓基质细胞的增殖和向成骨细胞分化的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：羊胎素对体外骨髓基质细胞的增殖和向成骨细胞分化的影响 1 1 器材 2 2 方法 3 3 结果 5 4 讨论 6 文2：淫羊藿苷对大鼠骨髓间充质干细胞向成骨细胞分化的影响 7 1 材料与方法 8 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 羊胎素对体外骨髓基质细胞的增殖和向成骨细胞分化的影响 文1：羊胎素对体外骨髓基质细胞的增殖和向成骨细胞分化的影响 Abstract：ObjectiveTo investigate the effects of placentin, the substance extract from lamb placenta, on SD rats marrow stroma cells ( MSCs) proliferation and osteogenic differentiation in vitro. MethodsProliferation of MSCs mediated by lamb placentin was measured by MTT assay. The alkaline phosphatase (ALP) activity of MSCs was detected by PNPP assay. Meanwhile, the cells osteogenic differentiation was examined by ALP growth of MSCs was obviously accelerated by the lamb placentin at the concentration of %, %, %, 5% (P); the activity of ALP of MSCs at the groups of % and 5% placentin containing osteoblast inducer was significantly higher than that of the blank group (P). However, the activity of ALP at the groups of % and 5% lamb Placentin without osteoblast inducer was significantly higher than the blank group and osteoblast inducer control group (P). The ALP staining cells at all placentin groups exhibited strong positive results. The colo of the cells at those groups were deeper than the inducer control group. ConclusionThe lamb placentin can remarkably increase the proliferation and induce the osteogenic differentiation of MSCs. Key words：Lamb placentin; Marrow stroma cells; Osteoblasts; Proliferation; Differentiation 羊胎素是利用 现代 生物技术从羊胎中提取的一种纯天然的生物活性物质，含有丰富的蛋白质，具有明显的抗辐射、抗衰老、抗肿瘤及提高人体免疫能力的作用［1~3］。本实验利用蛋白酶水解提取之羊胎素作用于体外培养的SD大鼠MSCs，观察羊胎素对MSCs增殖及向成骨细胞分化的影响，为羊胎素作为预防和 治疗 骨质疏松症提供依据。 1 器材 试剂与仪器DMEM（H、L糖）培养基，Gibco公司产品；胎牛血清，杭州四季清公司产品；胰蛋白酶、噻唑蓝、对硝基苯磷酸二钠、维生素C、地塞米松和β-甘油磷酸钠均为Sigma公司产品；Percoll液，Ameham pharmacia 公司产品；DMSO，江苏如皋富康试剂厂产品；其它试剂为广州化学试剂厂产品；CO2培养箱（美国NAPCO）；酶标仪（美国ELX800）；离心机（上海安亭仪器厂） 药物羊胎素（即羊胎水解液）由本院医药科技开发中心提供，取来自内蒙的健康羊胎，用现代工艺低温冷冻干燥制成。实验时取羊胎粉300 mg，在10倍量水中用蛋白酶水解8 h，除