肝细胞癌中缺氧诱导因子1α、热休克蛋白70的表达.doc

肝细胞癌中缺氧诱导因子-1α、热休克蛋白70的表达 【摘要】 目的 探讨缺氧诱导因子-1α(Hypoxiainduciblefactor-1α,HIF-1α)、热休克蛋白70(heat shock protein 70，HSP70)在肝细胞癌中的表达及其与癌转移的关系。方法　应用免疫组织化学技术检测48例肝细胞癌中HIF-1α、HSP70的表达。结果　48例肝细胞癌HIF-1α阳性率为70.8%,强表达10例,中度表达14例,弱表达10例,无表达14例,与正常对照组比较有显著性差异(P lt;0.05)。Ⅰ级、Ⅱ级、Ⅲ-Ⅳ级VEGF阳性细胞数三组比较有显著性差异(P lt;0.05)。患者癌转移者与无癌转移者HIF-1α、HSP70比较有显著性差异(P lt;0.01),HIF-2α表达与肝细胞癌恶性程度呈正相关(rr=0.726,,P lt;0.05)。HSP70与HIF-2α表达呈正相关( r=0.726,P lt;0.05)。结论 　HIF-2α与HSP70表达有相关性,HIF-1α、HSP70与癌转移有关, HIF-α与HSP70联合检测有助于判断肝细胞癌患者的预后。 【关键词】 肝细胞癌 缺氧诱导因子-1α 热休克蛋白 【Abstract】 Objective: To explore the expression of Hypoxiainduciblefactor-1α(HIF-1α) and heat shock protein 70(HSP70) in hepatocellular carcinoma, the relation between the expression and metastasis.Method: The expression of HIF-1αand HSP70 in 48 patients of liver carcinoma was detected via immunohistochemical analyses.Result:The HIF-1α-positive out of 48 patients was 70.8%,including 10 higher expression, 14medium expression, 10 lower expression and 14 non-expression patients,which was siginificantly higher than the controls(P lt;0.05). VEGF-positive cells among Ⅰ,Ⅱ and Ⅲ~Ⅳ grade were obviously different(P lt;0.05).The expression of HIF-1αand HSP70 of the patients with metastasis were siginificantly distinguished from those with no metastasis(P lt;0.01). and the expression of HIF-2α was positively associated with malignant degree(rr=0.726,P lt;0.05).The expression of HSP70 and HIF-2α was positively related(rr=0.726,P lt;0.05).Conclusion: The expression of HIF-2α is correlated with that of HSP70,and HIF-1αand HSP70 are associated with the metastasis. The combined detection of HIF-1αand HSP70 contribute to judge of the prognosis of hepatocellular carcinoma. 【Key words】hepatocellular carcinoma, Hypoxiainduciblefactor-1α, heat shock protein 70 HIF-1α（Hypoxia inducible factor-1α）是1992年发现的一种在缺氧条件下细胞产生的核蛋白,它与靶基因结合,促进其转录,使机体产生一系列缺氧适应反应[1]。现己证实，在任何氧浓度降低的情况下，如全身缺氧、脑动脉阻塞，都可能诱导HIF-1的快速大量表达[2]。肝细胞癌发生后，肿瘤的不断生长、体积增大,血供不足发生缺氧坏死,可诱导HIF-1α过度表达。因此，对以上过程在肝脏中对于下游基因的表达及与癌转移