一肾一夹肾血管性高血压动物模型的制作临床医学.docVIP

一肾一夹肾血管性高血压动物模型的制作临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：一肾一夹肾血管性高血压动物模型的制作临床医学 1 1 材料和方法 3 2 结果 4 3 讨论 5 文2：病证结合动物模型的制作评价与展望临床医学 6 1 病证结合模型的制备 7 2 病证结合模型的评价 10 3 展望 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 一肾一夹肾血管性高血压动物模型的制作临床医学 文1：一肾一夹肾血管性高血压动物模型的制作临床医学 Abstract： Objective To study the preparation of onekidney and oneclip (1K1C) renovascular hyperteion animal model. Methods The animal models of 1K1C renovascular hyperteion were established with SpragueDawley rats (200-220g per rat). The rats were randomly divided into four groups, . normal group, onekidney (1K) group, 1K1C group and 1K1C+BEA group (BEA=2bromoethylamine hydrobromide). The renal medulla was destroyed by BEA. Blood pressure was measured with tailcuff method by rat blood pressure meter of type RBP1. Results Compared with normal control group, the blood pressure in 1K group obviously increased (P), but four weeks later the blood pressure in both the groups did not achieved the standard of renovascular hyperteion. The blood pressure in 1K1C group began to increase one week later and achieved the threshold standard of renovascular hyperteion two weeks later. Compared with 1K1C group the blood pressure in 1K1C+BEA group increased more obviously after four weeks (P). Conclusion Our making method to rat model of renovascular hyperteion is simple and easy to operate. The success rate is about 80%, similar with those in the previous reports. Key words： onekidney one clip; hyperteion; animal model 肾血管性高血压(renovascular hyperteion)是指单侧或双侧肾动脉的主干或其分支狭窄，使肾血流量减少，导致肾缺血引起的高血压，为继发性高血压最常见的病因。我国肾血管性高血压占高血压人群的5%~7%[1]，肾血管的损害尚可引起受累肾尿的生成及内分泌功能异常。研究方法常以大鼠为实验动物，其动物模型制作有两种类型：二肾一夹(2K1C)型(一侧肾动脉狭窄，保留对侧肾脏)，其高血压早期系“肾素依赖型”；一肾一夹(1K1C)型(一侧肾动脉狭窄，对侧肾切除)，其高血压系“容量依赖型”。钳夹肾动脉的方法有丝线结扎、U型银夹。但丝线结扎对于以后去夹不利，而银夹不易制得，其大小不易控制。本研究 参考 傅继华等[2]的方法加以改进制作“Ω”形小夹，小夹内径 mm，制作简单，直径易控制，术后成功率较高，且高血压稳定。 1 材料和方法 实验材料 实验动物 成年雄性SD大鼠，体重200~220 g，福建医科大学实验动物中心提供，许可证号：SCXK(闽)20040002。 动脉夹子制作 将铝制易拉罐刮掉外表油漆，剪成长 cm，宽 mm 的长条