环磷酰胺对兔离体肺保护的作用.docVIP

环磷酰胺对兔离体肺保护的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：环磷酰胺对兔离体肺保护的作用 1 1 资料与方法 3 2 结果 4 3 讨论 5 文2：苦豆子总碱对家兔离体肺动脉血管作用机制的研究 6 1 材料与仪器 6 2 方法 7 3 结果 9 4 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 环磷酰胺对兔离体肺保护的作用 文1：环磷酰胺对兔离体肺保护的作用 The Protective Effect of Cyclophosphamide on Rabbits Isolated-lungs Preservation Abstract: OBJECTIVE To study the protective effects of cyclophosphamide on isolated lungs preservation using isolated-lungs reperfusion model of rabbit. METHODS The effects of cyclophosphamide on lung preservation were studied in 20 rabbits which were randomly divided into two groups (10 rabbits in each group). The experimental group was injected cyclophosphamide to cavitas abdominalis (50mg/kg), while the control group was injected same volume of normal saline (). The experiment were performed at 48 h after injection. Blood were draining out through right ventricular outlet completely, to make the rabbits heart arrest. The lungs of two groups were flushed and preserved with low potassium dextran solution after 1 hours warm ischemia and then reperfused after 4 hou of preservation. Pulmonary vascular resistance (PVR), lung resistance (LR) and blood gas were examined after 10 min，30min and 60 min of reperfusion. Lung water (LW) content was examined after 60 min of reperfusion. RESULTS It was found that:PVR was much lower in the experimental group than in the control group (P). While PO2 was much higher in the experimental group than in the control group(P).No deference was found in LR and LW between two groups. CONCLUSION Cyclophosphamide can improve the lung preservation perhaps by inhibiting the producing of inflammatory mediator. Key words： Cyclophosphamide;Rabbit；Isolated lungs preservation “离体肺保护”的研究对肺移植和心肺联合移植的成功有极其重要的意义。离体肺保护的主要研究包括：①肺保护液的成分、肺灌洗方法；②低温肺保护；③膨肺时所用气体成分［1］。目前的肺保护方法虽然能够满足常规临床使用，但人们仍然在探索更好的肺保护方法。本文在以上研究范围的基础上，从其它方面研究肺保护。肺保护液对离体肺的保护作用包括以下几个方面：①能够冲走存留在肺血管床内的血细胞，尤其是白细胞、血小板；②低温肺保护液能够降低肺组织温度；③有的肺保护液含有代谢底物，能够在低温无血情况下，有