大鼠离体肺再灌注模型的建立和改进.docVIP

大鼠离体肺再灌注模型的建立和改进 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大鼠离体肺再灌注模型的建立和改进 1 1 材料与方法 3 2 结 果 5 3 讨 论 5 文2：络气郁滞证大鼠动物模型的建立和评价 7 1 材料与仪器 8 2 方法 9 3 结果 10 4 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 大鼠离体肺再灌注模型的建立和改进 文1：大鼠离体肺再灌注模型的建立和改进 Establishment and Modification of Isolated Lung Traplantation and Abstract: OBJECTIVE To establish and modify of the isolated lung traplantation and reperfusion model in rats with a relatively less-invasive way. METHODS Angiocathete were ierted into femoral artery and jugular vein of the recipient rat to establish the controlled cross-circulatory. The venous cannula was connected to the pulmonary artery of the ex vivo lung block after preservation and cold storage to provide inflow，and Pulmonary effluent blood from the lung block was collected and returned to the support rat via the femoral artery for detoxification and deoxygenation. Each support rat was used to perfuse one lung block for at least one hour. The inflow sample to each lung block was analyzed for pH, pO2, pCO2, and Hb to follow alteratio in support rat physiology. RESULTS There were no statistical differences in pH, PvO2, PvCO2 or Hb （P）. CONCLUSION The support rat can deliver cotant systemic venous blood at stable physiologic paramete to the ex vivo lung block and this animal model can be used in research of lung traplantation and ischemic-reperfusion injury. Key words： Animal model; Lung traplantation;Donor;Ischemic-reperfusion injury; 目前，肺移植已成为一种 治疗 多种终末期肺部疾患的有效手段。但早期移植物功能障碍(primary graft failure，PGF)的发生始终是手术失败的主要原因，与移植后并发症发生率及病死率明显相关［1-2］。PGF病因复杂，降低PGF发生率的努力多集中在延长供体肺保存时间以及减轻供体肺缺血再灌注方面。原位大动物肺移植模型操作复杂，技术要求高，维护成本较高，且失败率高，小动物的体外循环(extracorporeal circulation,ECC)模型较少报道［3-4］。因此，建立一种简单可靠、重复性好、费用低廉的动物模型来评价肺保存及再灌注效果非常必要。本实验利用微创方法建立大鼠离体肺灌注模型，为开展大鼠肺移植手术供体肺保存及术后缺血再灌注损伤等研究提供了一种选择。 1 材料与方法 材料 清洁级纯种健康雄性SD大鼠（北京维通利华实验动物技术有限公司提供，实验动物机构许可证号：scxk(京)2002-0003），体重300～350 g。1％戊巴比妥钠，1000U/ml肝素化林格氏液，LPD液（low potassium dextran低钾右旋糖酐液）（自配）。呼吸机：DH-150型人工呼吸器（浙