体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型.docVIP

体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型 1 1 材料与方法 2 2 结果 3 3 讨论 5 文2：皮层脑电图监测下癫痫病灶切除术麻醉体会 7 2 结果 7 3 讨论 7 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 10 正文 体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型 文1：体重监测下硫代乙酰胺诱导豚鼠肝纤维化模型 [Abstract] Objective To observe guinea pig’s hepatic and systemic respoes to thioacetamide (TAA) during induction of hepatic fibrosis were monitored by weekly-weight changes,to explore the feasibility of inducing hepatic fibrosis model in guinea Fifty healthy male guinea pigs were divided randomly into three groups:control group(n=10); experimental group(n=40):the six-weeks group(n=20) and the nine-weeks group(n=20) received continuous administration of % (w/v) TAA+ % Vit-C in the drinking group (n=10) received the same concentration of % Vit-C in the drinking body weights were measured weekly once at same of liver about 5 μm thickness were stained with HE and Masson trichrome and Gomori staining was classified as:S0，S1，S2，S3， of all individuals in each group in the degree of hepatic fibrosis were After in-taking TAA,there was non-significant difference between experimental groups weekly,P individual’s body-weight has significant difference in experimental :control group were 10 cases;S1:TAA six-weeks group 4 cases;S2:TAA six-weeks group 14 cases,TAA nine-weeks group 5 cases;S3:TAA six-weeks group 1 cases,TAA nine-weeks group 9 cases;S4:TAA nine-weeks group 2 This experiment showed that thioacetamide can induce hepatic fibrosis model in guinea pigs; Weight monitoring is a non-invasive,simple,safe and effective mea of monitoring. [Key words] hepatic fibrosis;thioacetamide;guinea pigs 本实验拟以硫代乙酰胺(TAA)诱导豚鼠肝纤维化，通过体重变化监测结合肝脏病理的改变观察此模型的动态变化，初步分析豚鼠肝纤维化诱导过程中体重变化监测的作用，初步探索建立豚鼠肝纤维化模型的可行性、稳定性，寻找简便、有效、成模过程干扰少的监测手段。 1 材料与方法 实验分组与饲养 硫代乙酰胺(thioacetamide,TAA)分析纯(沈阳市试剂五厂，批号595181)。取体重在250～300 g(约3～4周龄)健康雄性豚鼠50只，随机分成两组：对照组10只;实验组40只(其中按TAA持续作用时间分为：TAA六周组20只，TAA九周组20只)。5只/笼，分笼饲养。室温控制在22 ℃左右，12 h明、12 h暗照明。对照组、实验组均予豚鼠基础饲料，不限