小脑顶核电刺激对大鼠脑梗死后皮层神经纤维再生的促进作用临床医学.docVIP

小脑顶核电刺激对大鼠脑梗死后皮层神经纤维再生的促进作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：小脑顶核电刺激对大鼠脑梗死后皮层神经纤维再生的促进作用临床医学 1 1 材料与方法 3 2 结 果 6 3 讨 论 7 文2：康复训练对大鼠脑梗死后Fos和Hsp70表达的影响 9 0 引言 10 1 材料和方法 10 2 结果 12 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 16 文章致谢（模板） 16 正文 小脑顶核电刺激对大鼠脑梗死后皮层神经纤维再生的促进作用临床医学 文1：小脑顶核电刺激对大鼠脑梗死后皮层神经纤维再生的促进作用临床医学 【Abstract】 Objective To study the effects of cerebella fastigial nucleus stimulation (F) on GAP43 mRNA expression in cerebral cortical tissue in post cerebral infarction SHR. Methods 68 of 3 monthold male spontaneous hyperteive rats (SH), weighing 200～250 g was employed. The middle cerebral artery occlusion (MCAO) model was duplicated. The total 60 min peistent F was given, then MCAO operation was conducted 24 h later in F group and in MCAO group animal only experienced the MCAO operation. The neurological deficiency was recorded every week following the MCAO. The experiment animal was decapitated at certain time points and brain tissue samples were taken for quantitative RT PCR analysis for GAP43 mRNA expression. Results 3 and 7 d after MCAO, the neurological deficiency score in F group (± and ± respectively) had no significant difference with those (± and ±) at same time point in MCAO group. It was decreased to ± at 14 d after MCAO in F group ,which was significantly different from ± in MCAO group (P＜). The level of of GAP43 mRNA expression in F group at 12 h and 3 d posterior of MCAO (± and ±,respectively) had no significant difference with those (± and ±) in MCAO group(P＞). At 7 d and 14 d time point, they reached the height of ± and ± respectively in F group, significantly higher than ± and ± in MCAO group(P＜). Conclusio F could significantly elevate the expression of GAP43 mRNA in post cerebral infarction brain tissue. It is possibly to have a role in post stroke behavior improvement. 【Key words】 Cerebral infarction；Cerebella fastigial nucleus stimulation;Neuronal plasticity;GAP43 小脑顶核电刺激（F）通过激活条件性神经源性神经保护机制，从而对缺血性脑损伤产