聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用（临床医学资料）.doc

聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用（临床医学资料） 文档信息 属性： F-01NHBE，doc格式，正文39338字。质优实惠，欢迎下载！ 适用： 作为医学资料、临床医学资料写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用 2 1 引 言 3 2 材料和方法 3 3 结果 6 4 讨论 7 文2：聚乙烯醇编织构建组织工程前交叉韧带的初步研究 9 1 材料与方法 9 2 结果 11 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用（临床医学资料） 文1：聚乙烯醇纺丝纤维编织在组织工程前交叉韧带支架材料中的应用 Abstract：To investigate the application feasibility of PVA scaffold modified by COL-I to cotruct anterior cruciate ligament tissue stripe scaffold with PVA spinning collagen cell line and human anterior cruciate ligament cells(HACL) were cultured and expanded in vitro and they were seeded into the scaffold which was braided with PVA, and PVA was modified by COL-I(PVA/COL). The growth situation of seeded cells into the scaffold was observed under the scanning electron microscope(SEM)，the mechanical properties of the scaffold was tested with the electronic showed that the porous structure of the scaffold facilitated the attachment and proliferation of NIH-3T3 cell line an HACL cells, and extracellular matrix was secreted. Cells adhered and spreaded better on the PVA/COL scaffold than on the PVA materials. COL-I could promote NIH-3T3 cell line to secrete extracellular matrix ,but no obvious effect on HACL was found. Pull tests showed that the flexibility of the material was strong，the peak load was ，the ultimate stress and elastic modulus was Mpa and Mpa could promote the attachment and proliferation of NIH-3T3 cell line and HACL cells,it also can promote NIH-3T3 cell line to secrete extracellular matrix, the scaffold braided with PVA/COL provide with good mechanical properties and biocompatibility,it is hopeful to become a useful scaffold in anterior cruciate ligament tissue engineering. Key words：Polyvinyl alcohol; Spinning braiding; Type I collagen; Anterior cruciate ligament; Tissue engineering 1 引 言 前交叉韧带（anterior cruciate lig